Literature DB >> 11271801

Purification and characterization of a thermostable alpha-amylase from Bacillus stearothermophilus.

K Chakraborty1, B K Bhattacharyya, S K Sen.   

Abstract

A soil isolate of Bacillus stearothermophilus was found to synthesize thermostable alpha-amylase. The enzyme was purified to homogeneity by ammonium sulfate fractionation and IECC on DEAE-cellulose column. The purified enzyme was considered to be a monomeric protein with a molar mass of 64 kDa, as determined by SDS-PAGE. The enzyme showed a wide range of pH tolerance and maximum activity at pH 7.0. The temperature tolerance was up to 100 degrees C with more than 90% catalytic activity; the maximum activity was observed at 50 degrees C. Divalent metal ions exhibited inhibitory effect on the enzyme activity. However, proteinase inhibitor did not react positively.

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Year:  2000        PMID： 11271801     DOI： 10.1007/bf02908945

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  6 in total

1.  Thermostable alpha-amylase of Bacillus stearothermophilus. IV. Amino-terminal and carboxyl-terminal amino acid analysis.

Authors:  L L CAMPBELL; P D CLEVELAND
Journal:  J Biol Chem       Date:  1961-11       Impact factor: 5.157

2.  The effects of surface adsorption on the thermal stability of proteins.

Authors:  B L Steadman; K C Thompson; C R Middaugh; K Matsuno; S Vrona; E Q Lawson; R V Lewis
Journal:  Biotechnol Bioeng       Date:  1992-06-05       Impact factor: 4.530

3.  Formation of extracellular alpha-amylase by Bacillus subtilis in relation to guanosine polyphosphates.

Authors:  R Wambutt; D Riesenberg; M Krüger; M Schultze
Journal:  Z Allg Mikrobiol       Date:  1984

4.  Thermostable alpha-amylase production using Bacillus licheniformis NRRL B14368.

Authors:  K Bose; D Das
Journal:  Indian J Exp Biol       Date:  1996-12       Impact factor: 0.818

5.  Microbial assimilation of hydrocarbons. II. Fatty acids derived from 1-alkenes.

Authors:  R Makula; W R Finnerty
Journal:  J Bacteriol       Date:  1968-06       Impact factor: 3.490

6.  Partial purification and properties of thermostable intracellular amylases from a thermophilic Bacillus sp. AK-2.

Authors:  R A Srivastava; S N Mathur; J N Baruah
Journal:  Acta Microbiol Pol       Date:  1984
  6 in total
  5 in total

1.  Purification of an alpha amylase from Aspergillus flavus NSH9 and molecular characterization of its nucleotide gene sequence.

Authors:  Kazi Muhammad Rezaul Karim; Ahmad Husaini; Ngieng Ngui Sing; Fazia Mohd Sinang; Hairul Azman Roslan; Hasnain Hussain
Journal:  3 Biotech       Date:  2018-03-29       Impact factor: 2.406

2.  Purification and Characterization of Thermostable and Detergent-Stable α-Amylase from Anoxybacillus sp. AH1.

Authors:  Ömer Acer; Fatma Matpan Bekler; Hemşe Pirinççioğlu; Reyhan Gül Güven; Kemal Güven
Journal:  Food Technol Biotechnol       Date:  2016-03       Impact factor: 3.918

3.  Thermostable glucose-tolerant glucoamylase produced by the thermophilic fungus Scytalidium thermophilum.

Authors:  A C Aquino; J A Jorge; H F Terenzi; M L Polizeli
Journal:  Folia Microbiol (Praha)       Date:  2001       Impact factor: 2.099

4.  Heterologous expression, biochemical characterization, and overproduction of alkaline α-amylase from Bacillus alcalophilus in Bacillus subtilis.

Authors:  Haiquan Yang; Long Liu; Jianghua Li; Guocheng Du; Jian Chen
Journal:  Microb Cell Fact       Date:  2011-10-07       Impact factor: 5.328

Review 5.  Marine Microbiological Enzymes: Studies with Multiple Strategies and Prospects.

Authors:  Yan Wang; Qinghao Song; Xiao-Hua Zhang
Journal:  Mar Drugs       Date:  2016-09-22       Impact factor: 5.118
  5 in total

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