Detection of rapidly labeled phosphatidic acid in lactating mammary gland of the intact rat.

Within 10 min following intravenous injections of phosphorus-32 labeled phosphoric acid into lactating rats, an intensely labeled pool of phosphatidic acid could be demonstarated in the mammary tissue. This finding provides supporting evidence for existence of the glycerol-3-phosphate pathway of milk triglyceride synthesis in the intact animal.