| Literature DB >> 1127018 |
L J Romrell, M R Coppe, D R Munro, S Ito.
Abstract
Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, which are larger than the other cell types, in fractions with purity of 75-95%. The homogeneity of cell fractions has been assessed by light and electron microscopy. The ability of the isolated cells to exclude the dye trypan blue, to incorporate labeled substrate, to consume oxygen, and to retain their structural integrity indicates that they are viable and still capable of functional activity.Entities:
Mesh：
Substances：
Year: 1975 PMID： 1127018 PMCID： PMC2109418 DOI： 10.1083/jcb.65.2.428
Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539