Literature DB >> 11266409

Application of a spectrophotometric method for the determination of post-antibiotic effect and comparison with viable counts in agar.

M C Domínguez1, M de La Rosa, M V Borobio.   

Abstract

The post-antibiotic effects of gentamicin and ciprofloxacin at 1x, 2x and 4x MIC on Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 were studied using a spectrophotometric method and the classic method of viable counts on agar as a reference. Monitoring of the growth kinetics was carried out by viability counting on the plate every hour and by means of the optical density of the cultures measured by spectrophotometry at a wavelength of 450 nm. No statistically significant differences were found between the results obtained with the spectrophotometric method and the reference method. The former method was much quicker, much easier to use and to replicate.

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Year:  2001        PMID: 11266409     DOI: 10.1093/jac/47.4.391

Source DB:  PubMed          Journal:  J Antimicrob Chemother        ISSN: 0305-7453            Impact factor:   5.790


  16 in total

1.  Antibacterial activity and cytotoxicity of PEGylated poly(amidoamine) dendrimers.

Authors:  Analette I Lopez; Rose Y Reins; Alison M McDermott; Barbara W Trautner; Chengzhi Cai
Journal:  Mol Biosyst       Date:  2009-07-03

2.  Postantibiotic effect and delay of regrowth in strains carrying mutations that save proteins or RNA.

Authors:  Marzia Dolcino; Alberto Zoratti; Eugenio A Debbia; Gian Carlo Schito; Anna Marchese
Journal:  Antimicrob Agents Chemother       Date:  2002-12       Impact factor: 5.191

3.  d-Methionine reduces tobramycin-induced ototoxicity without antimicrobial interference in animal models.

Authors:  Daniel J Fox; Morris D Cooper; Cristian A Speil; Melissa H Roberts; Susan C Yanik; Robert P Meech; Tim L Hargrove; Steven J Verhulst; Leonard P Rybak; Kathleen C M Campbell
Journal:  J Cyst Fibros       Date:  2015-07-10       Impact factor: 5.482

4.  In vitro antibacterial activity of Ibuprofen and acetaminophen.

Authors:  Ali Abdul Hussein S Al-Janabi
Journal:  J Glob Infect Dis       Date:  2010-05

5.  Quantitative analysis of gentamicin, azithromycin, telithromycin, ciprofloxacin, moxifloxacin, and oritavancin (LY333328) activities against intracellular Staphylococcus aureus in mouse J774 macrophages.

Authors:  Cristina Seral; Françoise Van Bambeke; Paul M Tulkens
Journal:  Antimicrob Agents Chemother       Date:  2003-07       Impact factor: 5.191

6.  Once-daily gentamicin administration for community-associated methicillin resistant Staphylococcus aureus in an in vitro pharmacodynamic model: preliminary reports for the advantages for optimizing pharmacodynamic index.

Authors:  Sun Woo Kim; Dong-Gun Lee; Su-Mi Choi; Chulmin Park; Jae-Cheol Kwon; Si-Hyun Kim; Sun Hee Park; Jung-Hyun Choi; Jin-Hong Yoo; Wan-Shik Shin
Journal:  Yonsei Med J       Date:  2010-09       Impact factor: 2.759

7.  Potential activity of the purine compounds caffeine and aminophylline on bacteria.

Authors:  Ali Abdul Hussein S Al-Janabi
Journal:  J Glob Infect Dis       Date:  2011-04

8.  Diversity of Multidrug Efflux Genes and Phenotypic Evaluation of the In vitro Resistance Dynamics of Clinical Staphylococcus Aureus Isolates Using Methicillin; a Model β-lactam.

Authors:  John F Antiabong; Marleen M Kock; Nontombi M Bellea; Marthie M Ehlers
Journal:  Open Microbiol J       Date:  2017-06-30

9.  Benzohydrazide and Phenylacetamide Scaffolds: New Putative ParE Inhibitors.

Authors:  Vidyasrilekha Yele; Bharat Kumar Reddy Sanapalli; Ashish D Wadhwani; Afzal Azam Mohammed
Journal:  Front Bioeng Biotechnol       Date:  2021-06-17

10.  In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates.

Authors:  Moustafa A El-Nakeeb; Hamida M Abou-Shleib; Amal M Khalil; Hoda G Omar; Omar M El-Halfawy
Journal:  Braz J Microbiol       Date:  2011-09-01       Impact factor: 2.476

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