Literature DB >> 11265760

The 3.7 A projection map of the glycerol facilitator GlpF: a variant of the aquaporin tetramer.

T Braun1, A Philippsen, S Wirtz, M J Borgnia, P Agre, W Kühlbrandt, A Engel, H Stahlberg.   

Abstract

GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Negative stain electron microscopy of solubilized GlpF protein revealed a tetrameric structure of approximately 80 A side length. Scanning transmission electron microscopy yielded a mass of 170 kDa, corroborating the tetrameric nature of GlpF. Reconstitution of GlpF in the presence of lipids produced highly ordered two-dimensional crystals, which diffracted electrons to 3.6 A resolution. Cryoelectron microscopy provided a 3.7 A projection map exhibiting a unit cell comprised of two tetramers. In projection, GlpF is similar to AQP1, the erythrocyte water channel. However, the major density minimum within each monomer is distinctly larger in GlpF than in AQP1.

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Year:  2000        PMID： 11265760      PMCID： PMC1084254          DOI： 10.1093/embo-reports/kvd022

Source DB:  PubMed          Journal:  EMBO Rep        ISSN： 1469-221X            Impact factor:   8.807


  32 in total

1.  Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography.

Authors:  P Ringler; M J Borgnia; H Stahlberg; P C Maloney; P Agre; A Engel
Journal:  J Mol Biol       Date:  1999-09-03       Impact factor: 5.469

2.  Molecular design of aquaporin-1 water channel as revealed by electron crystallography.

Authors:  H Li; S Lee; B K Jap
Journal:  Nat Struct Biol       Date:  1997-04

3.  Molecular structure of the water channel through aquaporin CHIP. The hourglass model.

Authors:  J S Jung; G M Preston; B L Smith; W B Guggino; P Agre
Journal:  J Biol Chem       Date:  1994-05-20       Impact factor: 5.157

4.  The three-dimensional structure of aquaporin-1.

Authors:  T Walz; T Hirai; K Murata; J B Heymann; K Mitsuoka; Y Fujiyoshi; B L Smith; P Agre; A Engel
Journal:  Nature       Date:  1997-06-05       Impact factor: 49.962

5.  The structure of aquaporin-1 at 4.5-A resolution reveals short alpha-helices in the center of the monomer.

Authors:  K Mitsuoka; K Murata; T Walz; T Hirai; P Agre; J B Heymann; A Engel; Y Fujiyoshi
Journal:  J Struct Biol       Date:  1999-12-01       Impact factor: 2.867

6.  Switch from an aquaporin to a glycerol channel by two amino acids substitution.

Authors:  V Lagrée; A Froger; S Deschamps; J F Hubert; C Delamarche; G Bonnec; D Thomas; J Gouranton; I Pellerin
Journal:  J Biol Chem       Date:  1999-03-12       Impact factor: 5.157

7.  Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.

Authors:  R Henderson; J M Baldwin; T A Ceska; F Zemlin; E Beckmann; K H Downing
Journal:  J Mol Biol       Date:  1990-06-20       Impact factor: 5.469

8.  Purified lens major intrinsic protein (MIP) forms highly ordered tetragonal two-dimensional arrays by reconstitution.

Authors:  L Hasler; T Walz; P Tittmann; H Gross; J Kistler; A Engel
Journal:  J Mol Biol       Date:  1998-06-19       Impact factor: 5.469

9.  Functional characterization of the Escherichia coli glycerol facilitator, GlpF, in Xenopus oocytes.

Authors:  C Maurel; J Reizer; J I Schroeder; M J Chrispeels; M H Saier
Journal:  J Biol Chem       Date:  1994-04-22       Impact factor: 5.157

10.  The three-dimensional structure of human erythrocyte aquaporin CHIP.

Authors:  T Walz; B L Smith; P Agre; A Engel
Journal:  EMBO J       Date:  1994-07-01       Impact factor: 11.598
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  7 in total

1.  Yeast Fps1 glycerol facilitator functions as a homotetramer.

Authors:  Sara E Beese-Sims; Jongmin Lee; David E Levin
Journal:  Yeast       Date:  2011-10-26       Impact factor: 3.239

2.  Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli.

Authors:  M J Borgnia; P Agre
Journal:  Proc Natl Acad Sci U S A       Date:  2001-02-20       Impact factor: 11.205

3.  Projection map of aquaporin-9 at 7 A resolution.

Authors:  Hector Viadiu; Tamir Gonen; Thomas Walz
Journal:  J Mol Biol       Date:  2006-12-20       Impact factor: 5.469

4.  Molecular dissection of water and glycerol permeability of the aquaglyceroporin from Plasmodium falciparum by mutational analysis.

Authors:  Eric Beitz; Slavica Pavlovic-Djuranovic; Masato Yasui; Peter Agre; Joachim E Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  2004-01-20       Impact factor: 11.205

5.  Molecular dynamics study of the archaeal aquaporin AqpM.

Authors:  Raul Araya-Secchi; J A Garate; David S Holmes; Tomas Perez-Acle
Journal:  BMC Genomics       Date:  2011-12-22       Impact factor: 3.969

6.  AQP9-induced cell cycle arrest is associated with RAS activation and improves chemotherapy treatment efficacy in colorectal cancer.

Authors:  Dandan Huang; Xingzhi Feng; Yiting Liu; Yanhong Deng; Hao Chen; Daici Chen; Lekun Fang; Yue Cai; Huanliang Liu; Lei Wang; Jianping Wang; Zihuan Yang
Journal:  Cell Death Dis       Date:  2017-06-22       Impact factor: 8.469

7.  Identification of key residues involved in Si transport by the aquaglyceroporins.

Authors:  Gabriel A Carpentier; Alexandre P Garneau; Andrée-Anne Marcoux; Micheline Noël; Rachelle Frenette-Cotton; Paul Isenring
Journal:  J Gen Physiol       Date:  2016-08-15       Impact factor: 4.086
  7 in total

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