F Wilhelm1, G Jendral, T Bredehom, G Duncker, D Wilhelms, A Kramer. 1. Klinik und Poliklinik für Augenheilkunde, Martin-Luther-Universität Halle-Wittenberg, Magdeburger Strasse 8, 06097 Halle. frank.wilhelm@medizin.uni-halle.de
Abstract
BACKGROUND: Microbiological examinations in eye banks have found an increased contamination rate in preservation media. We studied the effect of prolonged submersion time for decontaminating donor globes. MATERIALS AND METHODS: We retrospectively analyzed the primary contamination of conjunctival smears in 76 cornea donors. The submersion time of donor eyes in PVP iodine solution before preparation was prolonged from 1 min to 5 min, and the contamination rate of storage vessels was compared. RESULTS: In 13 of the 76 conjunctival smears we found no contamination. Before prolonging submersion time, the preservation medium was contaminated in 15 cases, but after 5 min no contamination was observed. CONCLUSION: Prolonging the submersion time of donor globes from 1 to 5 min was effective. The model presented here provides guidelines for existing eye banks as well as for those yet to be established.
BACKGROUND: Microbiological examinations in eye banks have found an increased contamination rate in preservation media. We studied the effect of prolonged submersion time for decontaminating donor globes. MATERIALS AND METHODS: We retrospectively analyzed the primary contamination of conjunctival smears in 76 cornea donors. The submersion time of donor eyes in PVPiodine solution before preparation was prolonged from 1 min to 5 min, and the contamination rate of storage vessels was compared. RESULTS: In 13 of the 76 conjunctival smears we found no contamination. Before prolonging submersion time, the preservation medium was contaminated in 15 cases, but after 5 min no contamination was observed. CONCLUSION: Prolonging the submersion time of donor globes from 1 to 5 min was effective. The model presented here provides guidelines for existing eye banks as well as for those yet to be established.
Authors: Tobias Röck; Johanna Landenberger; Michael Buhl; Efdal Yoeruek; Karl Ulrich Bartz-Schmidt; Matthias Bramkamp; Gunnar Blumenstock; Daniel Röck Journal: Medicine (Baltimore) Date: 2018-09 Impact factor: 1.817