Literature DB >> 11255255

Plasmids with the Cre-recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis.

H Y Steensma1, J J Ter Linde.   

Abstract

Two plasmids are described which can be used to remove the "loxP-markerMX-loxP" cassettes in strains lacking the ura3 mutation. Both contain the Cre-recombinase under control of the GAL1 promoter and the natMX cassette with the dominant marker nat, which gives yeasts resistance to the antibiotic ClonNat. pNatCre contains ARSH and CEN6 for maintenance in Saccharomyces cerevisiae. pKlNatCre has a Kluyveromyces lactis replication origin and centromere in addition. Copyright 2001 John Wiley & Sons, Ltd.

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Year:  2001        PMID： 11255255     DOI： 10.1002/yea.696

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  12 in total

Review 1.  PCR-based methods facilitate targeted gene manipulations and cloning procedures.

Authors:  Jürgen Wendland
Journal:  Curr Genet       Date:  2003-08-19       Impact factor: 3.886

2.  A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors:  U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

3.  Isolation by genetic and physiological characteristics of a fuel-ethanol fermentative Saccharomyces cerevisiae strain with potential for genetic manipulation.

Authors:  Eurípedes Alves da Silva Filho; Hélio Fernandes de Melo; Daiane Felberg Antunes; Scheila Karina Brito dos Santos; Alecsandra do Monte Resende; Diogo Ardaillon Simões; Marcos Antonio de Morais
Journal:  J Ind Microbiol Biotechnol       Date:  2005-10-15       Impact factor: 3.346

4.  Deletion of the glucose-6-phosphate dehydrogenase gene KlZWF1 affects both fermentative and respiratory metabolism in Kluyveromyces lactis.

Authors:  Michele Saliola; Gina Scappucci; Ilaria De Maria; Tiziana Lodi; Patrizia Mancini; Claudio Falcone
Journal:  Eukaryot Cell       Date:  2006-11-03

5.  Establishment of a Cre-loxP System Based on a Leaky LAC4 Promoter and an Unstable panARS Element in Kluyveromyces marxianus.

Authors:  Haiyan Ren; Anqi Yin; Pingping Wu; Huanyu Zhou; Jungang Zhou; Yao Yu; Hong Lu
Journal:  Microorganisms       Date:  2022-06-17

6.  Transcriptional neighborhoods regulate transcript isoform lengths and expression levels.

Authors:  Aaron N Brooks; Amanda L Hughes; Sandra Clauder-Münster; Leslie A Mitchell; Jef D Boeke; Lars M Steinmetz
Journal:  Science       Date:  2022-03-03       Impact factor: 63.714

7.  Intracellular NADPH levels affect the oligomeric state of the glucose 6-phosphate dehydrogenase.

Authors:  Michele Saliola; Angela Tramonti; Claudio Lanini; Samantha Cialfi; Daniela De Biase; Claudio Falcone
Journal:  Eukaryot Cell       Date:  2012-10-12

8.  Genome-wide metabolic (re-) annotation of Kluyveromyces lactis.

Authors:  Oscar Dias; Andreas K Gombert; Eugénio C Ferreira; Isabel Rocha
Journal:  BMC Genomics       Date:  2012-10-01       Impact factor: 3.969

9.  Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84.

Authors:  Janneke H M Teunissen; Marjolein E Crooijmans; Pepijn P P Teunisse; G Paul H van Heusden
Journal:  BMC Genomics       Date:  2017-09-06       Impact factor: 3.969

10.  An engineered cryptic Hxt11 sugar transporter facilitates glucose-xylose co-consumption in Saccharomyces cerevisiae.

Authors:  Hyun Yong Shin; Jeroen G Nijland; Paul P de Waal; René M de Jong; Paul Klaassen; Arnold J M Driessen
Journal:  Biotechnol Biofuels       Date:  2015-11-02       Impact factor: 6.040
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