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Literature DB >> 11254107

Differential scanning calorimetry of chromatin at different levels of condensation.

E Cardellini¹, S Cinelli, G L Gianfranceschi, G Onori, A Santucci, L Urbanelli.

Abstract

The thermal denaturation of calf thymus total chromatin and of fractions enriched in heterochromatin or euchromatin, has been investigated by differential scanning calorimetry and compared to that of calf thymus DNA and DNA-histone complexes. In our experimental conditions, chromatin melts in three thermal transitions: the main one, assigned to separation of the DNA double helix, occurs at 83 degrees C, while the other two occur at 63 degrees C and 74 degrees C. The data show that: (a) the transition enthalpy for denaturation of DNA in the total chromatin and in DNA-histone complexes is nearly the same as that of DNA in solution; (b) the transition at 63 degrees C is present in the thermogram of the heterocromatin enriched fraction, while it is completely absent in that of the euchromatin enriched one. The results suggest that this transition can be attributed to the higher order structures of heterochromatin.

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Year: 2000 PMID： 11254107 DOI： 10.1023/a:1007237930301

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

19 in total

1. Thermodynamics of condensation of nuclear chromatin. A differential scanning calorimetry study of the salt-dependent structural transitions.

Authors: B Cavazza; G Brizzolara; G Lazzarini; E Patrone; M Piccardo; P Barboro; S Parodi; A Pasini; C Balbi
Journal: Biochemistry Date: 1991-09-17 Impact factor: 3.162

2. In vitro simulation of solid-solid dehydration, rehydration, and solidification of trehalose dihydrate using thermal and vibrational spectroscopic techniques.

Authors: Shan-Yang Lin; Jui-Lung Chien
Journal: Pharm Res Date: 2003-12 Impact factor: 4.200

2 in total

Differential scanning calorimetry of chromatin at different levels of condensation.

1. Thermodynamics of condensation of nuclear chromatin. A differential scanning calorimetry study of the salt-dependent structural transitions.

2. High order DNA structure as inferred by optical fluorimetry and scanning calorimetry.

3. X-ray small angle scattering study of chromatin as a function of fiber length.

4. Photoreaction of furocoumarins with DNA is similarly inhibited by minor and major groove-interacting ligands.

5. A high melting (105 degrees C) form of chromatin characterizes the potential of cells for mitosis.

6. Protein-dependent conformational behavior of DNA in chromatin.

7. Calorimetric measurements of the transition enthalpy of DNA in aqueous urea solutions.

8. [Study of complexes of basic dyes with RNA].

9. Effects of salt concentration and H1 histone removal on the differential scanning calorimetry of nuclei.

10. Small peptides controlling transcription in vitro are bound to chromatin DNA.

1. Remodeling of heterochromatin induced by heavy metals in extreme old age.

2. In vitro simulation of solid-solid dehydration, rehydration, and solidification of trehalose dihydrate using thermal and vibrational spectroscopic techniques.