| Literature DB >> 1125221 |
H F Bienfait, J M Jacobs, E C Slater.
Abstract
1. The conditions under which mitochondria might catalyse a net reversal of oxidative phosphorylation are analysed. 2. Rat-liver mitochondria, incubated under such conditions, show a strongly diminished affinity for oxygen. 3. The velocity of respiration under these conditions is a hyperbolic function of the oxygen concentration. 4. The K-m for oxygen is less than 0.1 muM at low phosphate potential, irrespective of substrate, and 1-3 muM under reversal conditions. 5. The observed kinetics can be accounted for in a simple mechanism for cytochrome oxidase action.Entities:
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Year: 1975 PMID： 1125221 DOI： 10.1016/0005-2728(75)90166-8
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002