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Literature DB >> 11244079

A mutation in the 5' untranslated region increases stability of norA mRNA, encoding a multidrug resistance transporter of Staphylococcus aureus.

B Fournier¹, Q C Truong-Bolduc, X Zhang, D C Hooper.

Abstract

NorA, a multidrug efflux pump in Staphylococcus aureus, protects the cell from multiple drugs, including quinolones. The flqB mutation (T-->G) in the 5' untranslated region upstream of norA causes norA overexpression of 4.9-fold in cis, as measured in norA::blaZ fusions. The transcriptional initiation site of norA was unchanged in mutant and wild-type strains, but the half-life of norA mRNA was increased 4.8-fold in the flqB mutant compared to the wild-type strain. Computer-generated folding of the first 68 nucleotides of the norA transcript predicts an additional stem-loop and changes in a putative RNase III cleavage site in the flqB mutant.

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Year: 2001 PMID： 11244079 PMCID： PMC95146 DOI： 10.1128/JB.183.7.2367-2371.2001

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

29 in total

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