| Literature DB >> 11242108 |
S K Mahadevaiah1, J M Turner, F Baudat, E P Rogakou, P de Boer, J Blanco-Rodríguez, M Jasin, S Keeney, W M Bonner, P S Burgoyne.
Abstract
In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (gamma-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'.Entities:
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Year: 2001 PMID： 11242108 DOI： 10.1038/85830
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330