| Literature DB >> 11241027 |
N Hashimoto1, T Kawabe, T Hara, K Imaizumi, H Wakayama, H Saito, K Shimokata, Y Hasegawa.
Abstract
Erythromycin (EM) has an anti-inflammatory effect that may account for its clinical benefit in the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis. To investigate the mechanism of this anti-inflammatory effect, we studied the relationship between the concentration of EM and matrix metalloproteinase-9 (MMP-9) activity, which is important in cell migration. We showed that EM suppressed the gelatinolytic activity of U937 cell-derived MMP-9 by using gelatin zymography, showed that expressions of MMP-9 protein and MMP-9 mRNA were down-regulated by EM in a dose-dependent manner, and showed that U937 migration was also suppressed by EM. We also demonstrated that EM treatment suppressed the gelatinolytic activity of MMP-9 in spleen macrophages. These findings suggest that the suppressive effect of EM on MMP-9 activity is one of the anti-inflammatory mechanisms that inhibit the migration of inflammatory cells into the inflammatory site.Entities:
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Year: 2001 PMID： 11241027 DOI： 10.1067/mlc.2001.112726
Source DB: PubMed Journal: J Lab Clin Med ISSN： 0022-2143