Literature DB >> 11240199

Purification and partial characterization of a cholesterol oxidase from Streptomyces fradiae.

M T. Yazdi1, M Zahraei, K Aghaepour, N Kamranpour.   

Abstract

An extracellular cholesterol oxidase from Streptomyces fradiae (PTCC 1121) was purified in one step using DEAE-Sepharose. The purified enzyme had a molecular weight of 60 KDa. The optimum pH and temperature for activity was found to be 7 and 70 degrees C, respectively. This cholesterol oxidase was stable in pHs between 4-10 at 4 degrees C until 4 h. Thermal stability experiments showed that it has high stability and retains its full activity at 50 degrees C for 90 min. K(m) value for cholesterol oxidase was obtained to be about 7.06 x 10(-)(5) Mol.

Entities:  

Year:  2001        PMID： 11240199     DOI： 10.1016/s0141-0229(00)00337-9

Source DB:  PubMed          Journal:  Enzyme Microb Technol        ISSN： 0141-0229            Impact factor:   3.493


  8 in total

1.  Production, purification and characterization of cholesterol oxidase from a newly isolated Streptomyces sp.

Authors:  Ram Niwas; Vineeta Singh; Rajbir Singh; Divya Tripathi; C K M Tripathi
Journal:  World J Microbiol Biotechnol       Date:  2013-05-23       Impact factor: 3.312

2.  Cytotoxic activity of cholesterol oxidase produced by Streptomyces sp. AKHSS against cancerous cell lines: mechanism of action in HeLa cells.

Authors:  Kavitha Alapati; Savithri S Handanahal
Journal:  World J Microbiol Biotechnol       Date:  2021-07-21       Impact factor: 3.312

3.  Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42.

Authors:  Noura El-Ahmady El-Naggar; Nancy M El-Shweihy; Sara M El-Ewasy
Journal:  BMC Microbiol       Date:  2016-09-20       Impact factor: 3.605

4.  Purification, characterization and amino acid content of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102.

Authors:  Noura El-Ahmady El-Naggar; Sahar F Deraz; Hoda M Soliman; Nehal M El-Deeb; Nancy M El-Shweihy
Journal:  BMC Microbiol       Date:  2017-03-29       Impact factor: 3.605

5.  Extracellular cholesterol oxidase production by Streptomyces aegyptia, in vitro anticancer activities against rhabdomyosarcoma, breast cancer cell-lines and in vivo apoptosis.

Authors:  Noura El-Ahmady El-Naggar; Hoda M Soliman; Nancy M El-Shweihy
Journal:  Sci Rep       Date:  2018-02-09       Impact factor: 4.379

6.  Response Surface Methodology-Genetic Algorithm Based Medium Optimization, Purification, and Characterization of Cholesterol Oxidase from Streptomyces rimosus.

Authors:  Akanksha Srivastava; Vineeta Singh; Shafiul Haque; Smriti Pandey; Manisha Mishra; Arshad Jawed; P K Shukla; P K Singh; C K M Tripathi
Journal:  Sci Rep       Date:  2018-07-19       Impact factor: 4.379

7.  Purification and characterisation of the extracellular cholesterol oxidase enzyme from Enterococcus hirae.

Authors:  Hany M Yehia; Wesam A Hassanein; Shimaa M Ibraheim
Journal:  BMC Microbiol       Date:  2015-09-14       Impact factor: 3.605

8.  Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain.

Authors:  Elsayed Ahmed Elsayed; Nayera Ahmed Abdelwahed
Journal:  Biomed Res Int       Date:  2020-03-31       Impact factor: 3.411
  8 in total

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