BACKGROUND AND PURPOSE: A high degree of proteinuria has been reported in stroke-prone spontaneously hypertensive rats (SHRSP). We studied the effect of salt loading on the detailed protein pattern of serum and urine in 3 rat strains: Wistar-Kyoto, spontaneously hypertensive rats, and SHRSP, an inbred animal model for a complex form of cerebrovascular disorder resembling the human disease. METHODS: Rats were given a permissive diet and received 1% NaCl in drinking water. The protein pattern in body fluids was assessed over time by 2-dimensional electrophoretic analysis. Brain alterations were monitored by MRI and histology. RESULTS: Several proteins were excreted in urine after weeks of treatment and in advance of stroke: transferrin, hemopexin, albumin, alpha(2)-HS-glycoprotein, kallikrein-binding protein, alpha(1)-antitrypsin, Gc-globulin, and transthyretin. Markers of an inflammatory response, including very high levels of thiostatin, were detected in the serum of SHRSP at least 4 weeks before a stroke occurred. CONCLUSIONS: In SHRSP subjected to salt loading, an atypical inflammatory condition and widespread alterations of vascular permeability developed before the appearance of anomalous features in the brain detected by MRI. Urinary concentrations of each of the excreted serum proteins correlated positively with time before stroke occurred.
BACKGROUND AND PURPOSE: A high degree of proteinuria has been reported in stroke-prone spontaneously hypertensiverats (SHRSP). We studied the effect of salt loading on the detailed protein pattern of serum and urine in 3 rat strains: Wistar-Kyoto, spontaneously hypertensiverats, and SHRSP, an inbred animal model for a complex form of cerebrovascular disorder resembling the human disease. METHODS:Rats were given a permissive diet and received 1% NaCl in drinking water. The protein pattern in body fluids was assessed over time by 2-dimensional electrophoretic analysis. Brain alterations were monitored by MRI and histology. RESULTS: Several proteins were excreted in urine after weeks of treatment and in advance of stroke: transferrin, hemopexin, albumin, alpha(2)-HS-glycoprotein, kallikrein-binding protein, alpha(1)-antitrypsin, Gc-globulin, and transthyretin. Markers of an inflammatory response, including very high levels of thiostatin, were detected in the serum of SHRSP at least 4 weeks before a stroke occurred. CONCLUSIONS: In SHRSP subjected to salt loading, an atypical inflammatory condition and widespread alterations of vascular permeability developed before the appearance of anomalous features in the brain detected by MRI. Urinary concentrations of each of the excreted serum proteins correlated positively with time before stroke occurred.
Authors: Jim C Oates; Sanju Varghese; Alison M Bland; Timothy P Taylor; Sally E Self; Romesh Stanislaus; Jonas S Almeida; John M Arthur Journal: Kidney Int Date: 2005-12 Impact factor: 10.612
Authors: Limor Raz; Kiran Bhaskar; John Weaver; Sandro Marini; Quanguang Zhang; Jeffery F Thompson; Candice Espinoza; Sulaiman Iqbal; Nicole M Maphis; Lea Weston; Laurel O Sillerud; Arvind Caprihan; John C Pesko; Erik B Erhardt; Gary A Rosenberg Journal: Neurobiol Dis Date: 2018-07-25 Impact factor: 5.996
Authors: Sun Shin Yi; In Koo Hwang; Myung Sun Chun; Yo Na Kim; Il Yong Kim; In Se Lee; Je Kyung Seong; Yeo Sung Yoon Journal: Neurochem Res Date: 2008-08-29 Impact factor: 3.996