Literature DB >> 112369

Simple method to detect virus-specific IgM antibodies in patients' serum samples after immunosorption of immunoglobulins G and A.

H P Geisen, R Frank, H W Doerr, G Enders.   

Abstract

By absorption with protein A and specific immunosorption using insolubilized adsorbentia (CPG), a method was developed for rapid elimination of IgG and IgA from patients' serum samples. This test technique was examined in the diagnosis of rubella infections, i.e., rubella-specific IgM antibodies. The procedure has proven to be as reliable as determinations carried out with IgM fractions separated on a sucroe density gradient.

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Year:  1979        PMID: 112369     DOI: 10.1007/bf02123557

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN: 0300-8584            Impact factor:   3.402


  10 in total

1.  Simple detection of fluorescent stained IgM in sucrose gradients: demonstration of virus-specific IgM.

Authors:  H Schmitz; C M Krainick-Rechert
Journal:  Intervirology       Date:  1974       Impact factor: 1.763

2.  Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies.

Authors:  T Vesikari; A Vaheri
Journal:  Br Med J       Date:  1968-01-27

3.  A rountine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus.

Authors:  J Ankerst; P Christensen; L Kjellén; G Kronvall
Journal:  J Infect Dis       Date:  1974-09       Impact factor: 5.226

4.  Serum IgM and IgG responses in postnatally acquired rubella.

Authors:  J M Best; J E Banatvala; D Watson
Journal:  Lancet       Date:  1969-07-12       Impact factor: 79.321

5.  Persistence of rubellavirus-specific immunoglobulin M and immunoglobulin A antibodies: investigation of successive serum samples with lowered immunoglobulin G concentration.

Authors:  A Hornsleth; J Leerhoy; P Grauballe; H Spanggaard
Journal:  Infect Immun       Date:  1975-04       Impact factor: 3.441

6.  [Rubella virus hemagglutination inhibition IgM antibodies: the method of absorption of IgG by staphylococcal protein A as compared with density gradient ultracentrifugation (author's transl)].

Authors:  M Roggendorf; K E Schneweis; M H Wolff
Journal:  Zentralbl Bakteriol Orig A       Date:  1976-08

7.  Absorption of blocking activity from human tumor-bearer sera by Staphylococcus aureus, Cowan I.

Authors:  G Steele; J Ankerst; H O Sjögren; J Vang; O Lannerstad
Journal:  Int J Cancer       Date:  1975-02-15       Impact factor: 7.396

8.  Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies.

Authors:  H Schmitz; H Shimizu; D Kampa; H W Doerr
Journal:  J Clin Microbiol       Date:  1975-02       Impact factor: 5.948

9.  Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column.

Authors:  J D Gupta; V Peterson; M Stout; A M Murphy
Journal:  J Clin Pathol       Date:  1971-09       Impact factor: 3.411

10.  Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.

Authors:  A Bürgin-Wolff; R Hernandez; M Just
Journal:  Lancet       Date:  1971-12-11       Impact factor: 79.321

  10 in total
  2 in total

1.  Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella and Treponema pallidum.

Authors:  U Huschka; J Maier; E W Rauterberg; H W Doerr
Journal:  Eur J Clin Microbiol       Date:  1982-04       Impact factor: 3.267

2.  Human cytomegalovirus infection: recent developments in diagnosis and epidemiology.

Authors:  H W Doerr; R Braun; K Munk
Journal:  Klin Wochenschr       Date:  1985-03-15
  2 in total

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