AIM: To investigate the effect of vagus nerve on coordinating the motion of gallbladder (GB) and sphincter of Oddi (SO) in the interdigestive period in rabbits. METHODS: Fasted for 15 h-18 h, but allowed to drink water, the rabbib were anesthetized with urethane (1.0 mg/kg, i.v.). In order to measure GB pressure, a frog bladder filled with normal saline was put into GB and connected to a transducer (TP-200T). Myoelectric signals of SO was recorded by a pair of copper electrodes. RESULTS: In the interdigestive period, phasic contractions of GB (PCGB) and clusters of spike potentials of SO (CSPSO) was 1:1 correlated (Y = 0.962X + 0.587, r = 0.982, P < 0.01). That is, every PCGB was accompanied by one CSPSO. Microinjection of thyrotropin-releasing hormone (TRH, 0.8 nmol, 1 microliter) or monosodium glutamate (MSG, 2 mumol, 1 microliter) into dorsal vagal complex (DVC) enhanced the motility of GB and SO, and the 1:1 temporal relation between PCGB and CSPSO still existed. Vagotomy or intravenous injection of atropine inhibited the PCGB and the spike potentials of SO, and the 1:1 correlation between PCGB and CSPSO disappeared. The spike bursts of SO did not respond to the artificial rise of gallbladder pressure. CONCLUSION: In the interdigestive period in rabbits, gallbladder and SO contract and relax rhythmically and simultaneously. This eoordinated motion between GB and SO is controlled by DVC via vagus nerve and peripheral M receptors.
AIM: To investigate the effect of vagus nerve on coordinating the motion of gallbladder (GB) and sphincter of Oddi (SO) in the interdigestive period in rabbits. METHODS: Fasted for 15 h-18 h, but allowed to drink water, the rabbib were anesthetized with urethane (1.0 mg/kg, i.v.). In order to measure GB pressure, a frog bladder filled with normal saline was put into GB and connected to a transducer (TP-200T). Myoelectric signals of SO was recorded by a pair of copper electrodes. RESULTS: In the interdigestive period, phasic contractions of GB (PCGB) and clusters of spike potentials of SO (CSPSO) was 1:1 correlated (Y = 0.962X + 0.587, r = 0.982, P < 0.01). That is, every PCGB was accompanied by one CSPSO. Microinjection of thyrotropin-releasing hormone (TRH, 0.8 nmol, 1 microliter) or monosodium glutamate (MSG, 2 mumol, 1 microliter) into dorsal vagal complex (DVC) enhanced the motility of GB and SO, and the 1:1 temporal relation between PCGB and CSPSO still existed. Vagotomy or intravenous injection of atropine inhibited the PCGB and the spike potentials of SO, and the 1:1 correlation between PCGB and CSPSO disappeared. The spike bursts of SO did not respond to the artificial rise of gallbladder pressure. CONCLUSION: In the interdigestive period in rabbits, gallbladder and SO contract and relax rhythmically and simultaneously. This eoordinated motion between GB and SO is controlled by DVC via vagus nerve and peripheral M receptors.