M Zheng1, Y Ruan, Z Wu. 1. Department of Pathology, Jiujiang Medical College, Jiujiang 332000, China.
Abstract
OBJECTIVE: To study the correlation between tumor cell expression of TGF beta and the amount of surrounding mast cells in hepatocarcinogenesis. METHODS: Hepatocarcinoma was induced by diethylnitrosamine (DENA) in rats. The expression of TGF beta was examined immunohistochemically, and mast cells were identified by Alcian blue staining. The results were undergone cell imaging analysis. RESULTS: Positive expression of TGF beta was observed in the cytoplasm of the hepatocarcinoma cells. The intensity of expression differd in various groups of experiment. There was a positive correlation between the intensity of the TGF beta expression in hepatocarcinoma cells and the amount of mast cells surrounding the tumor nodules (P < 0.01). CONCLUSION: Mast cell infiltration in DENA-induced rat hepatoma may be due to the chemotactic effect of TGF beta expressed in tumor cells.
OBJECTIVE: To study the correlation between tumor cell expression of TGF beta and the amount of surrounding mast cells in hepatocarcinogenesis. METHODS: Hepatocarcinoma was induced by diethylnitrosamine (DENA) in rats. The expression of TGF beta was examined immunohistochemically, and mast cells were identified by Alcian blue staining. The results were undergone cell imaging analysis. RESULTS: Positive expression of TGF beta was observed in the cytoplasm of the hepatocarcinoma cells. The intensity of expression differd in various groups of experiment. There was a positive correlation between the intensity of the TGF beta expression in hepatocarcinoma cells and the amount of mast cells surrounding the tumor nodules (P < 0.01). CONCLUSION: Mast cell infiltration in DENA-induced rathepatoma may be due to the chemotactic effect of TGF beta expressed in tumor cells.