| Literature DB >> 11233609 |
N M Tsai1, T L Cheng, S R Roffler.
Abstract
An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. PEG-modified beta-glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the linker used for PEG attachment.Entities:
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Year: 2001 PMID： 11233609 DOI： 10.2144/01302rr03
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993