Literature DB >> 11233604

High-throughput AFLP analysis using infrared dye-labeled primers and an automated DNA sequencer.

A A Myburg1, D L Remington, D M O'Malley, R R Sederoff, R W Whetten.   

Abstract

Amplified fragment length polymorphism (AFLP) analysis is currently the most powerful and efficient technique for the generation of large numbers of anonymous DNA markers in plant and animal genomes. We have developed a protocol for high-throughput AFLP analysis that allows up to 70,000 polymorphic marker genotype determinations per week on a single automated DNA sequencer. This throughput is based on multiplexed PCR amplification of AFLP fragments using two different infrared dyelabeled primer combinations. The multiplexed AFLPs are resolved on a two-dye, model 4200 LI-COR automated DNA sequencer, and the digital images are scored using semi-automated scoring software specifically designed for complex AFLP banding patterns (AFLP-Quantar). Throughput is enhanced by using high-quality genomic DNA templates obtained by a 96-well DNA isolation procedure.

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Substances：

Year:  2001        PMID： 11233604     DOI： 10.2144/01302tt04

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  19 in total

1.  A composite linkage map from two crosses for the species complex Picea mariana x Picea rubens and analysis of synteny with other Pinaceae.

Authors:  Betty Pelgas; Jean Bousquet; Stéphanie Beauseigle; Nathalie Isabel
Journal:  Theor Appl Genet       Date:  2005-11-10       Impact factor: 5.699

2.  Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis.

Authors:  H-w Cai; Z-s Gao; N Yuyama; N Ogawa
Journal:  Mol Genet Genomics       Date:  2003-03-26       Impact factor: 3.291

3.  Three QTLs for Botrytis cinerea resistance in tomato.

Authors:  Richard Finkers; Petra van den Berg; Ralph van Berloo; Arjen ten Have; Adriaan W van Heusden; Jan A L van Kan; Pim Lindhout
Journal:  Theor Appl Genet       Date:  2006-11-30       Impact factor: 5.699

4.  Genetics of postzygotic isolation in Eucalyptus: whole-genome analysis of barriers to introgression in a wide interspecific cross of Eucalyptus grandis and E. globulus.

Authors:  Alexander A Myburg; Claus Vogl; A Rod Griffin; Ronald R Sederoff; Ross W Whetten
Journal:  Genetics       Date:  2004-03       Impact factor: 4.562

5.  Comparative genetic linkage maps of Eucalyptus grandis, Eucalyptus globulus and their F1 hybrid based on a double pseudo-backcross mapping approach.

Authors:  A A Myburg; A R Griffin; R R Sederoff; R W Whetten
Journal:  Theor Appl Genet       Date:  2003-07-01       Impact factor: 5.699

6.  Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L.).

Authors:  Shiqiang Liu; Liang Xu; Zhiqi Jia; Yong Xu; Qing Yang; Zhangjun Fei; Xiangyang Lu; Huiming Chen; Sanwen Huang
Journal:  Theor Appl Genet       Date:  2008-07-16       Impact factor: 5.699

7.  High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae).

Authors:  Lucy Gonthier; Christelle Blassiau; Monika Mörchen; Thierry Cadalen; Matthieu Poiret; Theo Hendriks; Marie-Christine Quillet
Journal:  Theor Appl Genet       Date:  2013-05-21       Impact factor: 5.699

8.  Genetic analysis of morphological traits in a new, versatile, rapid-cycling Brassica rapa recombinant inbred line population.

Authors:  Hedayat Bagheri; Mohamed El-Soda; Inge van Oorschot; Corrie Hanhart; Guusje Bonnema; Tanja Jansen-van den Bosch; Rolf Mank; Joost J B Keurentjes; Lin Meng; Jian Wu; Maarten Koornneef; Mark G M Aarts
Journal:  Front Plant Sci       Date:  2012-08-16       Impact factor: 5.753

9.  Comparing EST-based genetic maps between Pinus sylvestris and Pinus taeda.

Authors:  P Komulainen; G R Brown; M Mikkonen; A Karhu; M R García-Gil; D O'Malley; B Lee; D B Neale; O Savolainen
Journal:  Theor Appl Genet       Date:  2003-06-25       Impact factor: 5.699

10.  A sequence-anchored genetic linkage map for the moss, Physcomitrella patens.

Authors:  Yasuko Kamisugi; Mark von Stackelberg; Daniel Lang; Matthew Care; Ralf Reski; Stefan A Rensing; Andrew C Cuming
Journal:  Plant J       Date:  2008-07-23       Impact factor: 6.417
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