C Sobarzo1, E Bustos-Obregón. 1. Faculty of Basic Sciences, Catholic University of Temuco and Biology of Reproduction Unit, Program of Morphology, ICBM, Santiago, Chile.
Abstract
AIM: To investigate the toxic effect of a single injection of the organophosphorous agropesticide, parathion, on spermatogenesis in immature male mice. METHODS: Seven-day old mice received a single injection of parathion intraperitoneally at a dose of 1/3 LD50. The epididymal sperm count, sperm morphology and chromatin thermal stability were analyzed 28 and 50 days after injection. RESULTS: Sperm counts were decreased and teratozoospermia and thermal denaturation of DNA increased after parathion injection. Sperm parameters were changed to a greater extent in younger animals, denoting a higher lability of spermatogenic process at its beginning. The damages could recover a long time after parathion administration. CONCLUSION: Organophosphorous agropesticides are testicular toxicants, eliciting reversible cytotoxic and cytogenetic alterations in germ cells.
AIM: To investigate the toxic effect of a single injection of the organophosphorous agropesticide, parathion, on spermatogenesis in immature male mice. METHODS: Seven-day old mice received a single injection of parathion intraperitoneally at a dose of 1/3 LD50. The epididymal sperm count, sperm morphology and chromatin thermal stability were analyzed 28 and 50 days after injection. RESULTS: Sperm counts were decreased and teratozoospermia and thermal denaturation of DNA increased after parathion injection. Sperm parameters were changed to a greater extent in younger animals, denoting a higher lability of spermatogenic process at its beginning. The damages could recover a long time after parathion administration. CONCLUSION:Organophosphorous agropesticides are testicular toxicants, eliciting reversible cytotoxic and cytogenetic alterations in germ cells.
Authors: Ferdinand Ngoula; Pierre Watcho; Marie-Chantal Dongmo; Augustave Kenfack; Pierre Kamtchouing; Joseph Tchoumboué Journal: Afr Health Sci Date: 2007-03 Impact factor: 0.927