PURPOSE: We gave high single doses of external radiation to normal vessels to produce vascular injury and establish the dose tolerance in an animal model. We also performed immunohistochemical staining for macrophages and smooth muscle cells to assess qualitative changes in their populations. METHODS: Following direct bilateral inguinal cutdown in New Zealand white rabbits, single doses of 15, 20 and 30 Gy were delivered to one vessel. At predetermined time intervals following treatment, the animals underwent angiography and were sacrificed. Both systems were harvested and analyzed, and their luminal and medial areas compared. RESULTS: No statistically significant differences were found between any treatment vessel and its contralateral control at any time point. In addition, no alterations in subintimal or medial content of macrophages or smooth muscle cells were observed. CONCLUSIONS: Our data suggest that single radiation doses as high as 30 Gy appear to be well tolerated in the normal, uninjured rabbit vessel over a 6-month follow-up. However, the use of a diseased vessel model and longer follow-up times need to be studied to provide a better clinical understanding of the basic radiobiology of this technique.
PURPOSE: We gave high single doses of external radiation to normal vessels to produce vascular injury and establish the dose tolerance in an animal model. We also performed immunohistochemical staining for macrophages and smooth muscle cells to assess qualitative changes in their populations. METHODS: Following direct bilateral inguinal cutdown in New Zealand white rabbits, single doses of 15, 20 and 30 Gy were delivered to one vessel. At predetermined time intervals following treatment, the animals underwent angiography and were sacrificed. Both systems were harvested and analyzed, and their luminal and medial areas compared. RESULTS: No statistically significant differences were found between any treatment vessel and its contralateral control at any time point. In addition, no alterations in subintimal or medial content of macrophages or smooth muscle cells were observed. CONCLUSIONS: Our data suggest that single radiation doses as high as 30 Gy appear to be well tolerated in the normal, uninjured rabbit vessel over a 6-month follow-up. However, the use of a diseased vessel model and longer follow-up times need to be studied to provide a better clinical understanding of the basic radiobiology of this technique.
Authors: Jaden D Evans; Daniel R Gomez; Arya Amini; Neal Rebueno; Pamela K Allen; Mary K Martel; Justin M Rineer; Kie Kian Ang; Sarah McAvoy; James D Cox; Ritsuko Komaki; James W Welsh Journal: Radiother Oncol Date: 2013-02-28 Impact factor: 6.280