Literature DB >> 11229403

Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.

C Wiese1, A Wilde, M S Moore, S A Adam, A Merdes, Y Zheng.   

Abstract

The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-beta. We show that importin-beta is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-beta and NuMA. Importin-beta therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.

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Year:  2001        PMID： 11229403     DOI： 10.1126/science.1057661

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  127 in total

Review 1.  Transport into and out of the nucleus.

Authors:  I G Macara
Journal:  Microbiol Mol Biol Rev       Date:  2001-12       Impact factor: 11.056

2.  Antisense expression of an Arabidopsis ran binding protein renders transgenic roots hypersensitive to auxin and alters auxin-induced root growth and development by arresting mitotic progress.

Authors:  S H Kim; D Arnold; A Lloyd; S J Roux
Journal:  Plant Cell       Date:  2001-12       Impact factor: 11.277

3.  The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity.

Authors:  Michael J E Krien; Robert R West; Ulrik P John; Kalli Koniaras; J Richard McIntosh; Matthew J O'Connell
Journal:  EMBO J       Date:  2002-04-02       Impact factor: 11.598

4.  Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.

Authors:  Peter Askjaer; Vincent Galy; Eva Hannak; Iain W Mattaj
Journal:  Mol Biol Cell       Date:  2002-12       Impact factor: 4.138

5.  Part of Ran is associated with AKAP450 at the centrosome: involvement in microtubule-organizing activity.

Authors:  Guy Keryer; Barbara Di Fiore; Claude Celati; Karl Ferdinand Lechtreck; Mette Mogensen; Annie Delouvee; Patrizia Lavia; Michel Bornens; Anne-Marie Tassin
Journal:  Mol Biol Cell       Date:  2003-07-11       Impact factor: 4.138

Review 6.  Nucleo-cytoplasmic partitioning of proteins in plants: implications for the regulation of environmental and developmental signalling.

Authors:  Thomas Merkle
Journal:  Curr Genet       Date:  2003-10-02       Impact factor: 3.886

7.  Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM.

Authors:  Haruko Ideguchi; Atsuhisa Ueda; Masatsugu Tanaka; Jun Yang; Takashi Tsuji; Shigeru Ohno; Eri Hagiwara; Akiko Aoki; Yoshiaki Ishigatsubo
Journal:  Biochem J       Date:  2002-10-01       Impact factor: 3.857

8.  Importin alpha-regulated nucleation of microtubules by TPX2.

Authors:  Christoph A Schatz; Rachel Santarella; Andreas Hoenger; Eric Karsenti; Iain W Mattaj; Oliver J Gruss; Rafael E Carazo-Salas
Journal:  EMBO J       Date:  2003-05-01       Impact factor: 11.598

9.  Analysis of the small GTPase gene superfamily of Arabidopsis.

Authors:  Vanessa Vernoud; Amy C Horton; Zhenbiao Yang; Erik Nielsen
Journal:  Plant Physiol       Date:  2003-03       Impact factor: 8.340

10.  Importin alpha/beta and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal.

Authors:  Stephanie C Ems-McClung; Yixian Zheng; Claire E Walczak
Journal:  Mol Biol Cell       Date:  2003-09-17       Impact factor: 4.138
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