| Literature DB >> 11223254 |
G P Lin Cereghino1, J Lin Cereghino, A J Sunga, M A Johnson, M Lim, M A Gleeson, J M Cregg.
Abstract
We describe the isolation and characterization of three new biosynthetic genes-ARG4, ADE1, and URA3-from the methylotrophic yeast Pichia pastoris. The predicted products of the genes share significant sequence similarity to their Saccharomyces cerevisiae counterparts, namely argininosuccinate lyase, PR-aminoimidazolesuccinocarboxamide synthase, and orotidine-5'-phosphate decarboxylase, respectively. Along with the previously described HIS4 gene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P. pastoris. In addition, we have constructed a series of host strains containing all possible combinations of ade1, arg4, his4, and ura3 auxotrophies to be used with these new vectors.Entities:
Mesh：
Substances：
Year: 2001 PMID： 11223254 DOI： 10.1016/s0378-1119(00)00576-x
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688