C J Earley1, K Hyland, R P Allen. 1. Department of Neurology, Johns Hopkins Bayview Medical Center, Baltimore, Maryland 21224, USA.
Abstract
PURPOSE: To evaluate the role of CNS dopaminergic systems in Restless Legs Syndrome (RLS), homovanillic acid (HVA), tetrahydrobiopterin (BH4), and neopterin (NEOP), were assayed in CSF from RLS patients. The serotonin metabolite, 5-hydroxyindoleacetic acid (5-HIAA), was also measured. METHODS: CSF was taken from 16 RLS patients after 2 weeks off medication and from 14 control subjects. The CSF metabolites were determined using HPLC techniques. RESULTS: There was no significant difference in HVA or 5-HIAA, but NEOP and BH4 were higher in RLS patients. The RLS group was significantly older than the control group (64.2 +/- 9.2 years vs. 51.4 +/- 6.3 years; P < 0.001). A multiple regression analysis showed a strong correlation between age and 5-HIAA (r = 0.46, P = 0.04) and between age and NEOP (r = 0.61, P < 0.01). To eliminate the potential error created by the age difference between groups, an age-adjusted subgroup of RLS and control subjects were compared. There was still no difference found for HVA; however, 5-HIAA was now significantly lower (P < 0.01) in the RLS subgroup. Age-adjustment eliminated the differences previously found for NEOP, (P = 0.12), but BH4 continued to remain higher in the RLS group (P < 0.01). CONCLUSION: Differences in CSF HVA concentrations were not found. The changes in 5-HIAA and BH4 are of unclear clinical significance and require further assessment with appropriate age-matched controls.
PURPOSE: To evaluate the role of CNS dopaminergic systems in Restless Legs Syndrome (RLS), homovanillic acid (HVA), tetrahydrobiopterin (BH4), and neopterin (NEOP), were assayed in CSF from RLS patients. The serotonin metabolite, 5-hydroxyindoleacetic acid (5-HIAA), was also measured. METHODS:CSF was taken from 16 RLS patients after 2 weeks off medication and from 14 control subjects. The CSF metabolites were determined using HPLC techniques. RESULTS: There was no significant difference in HVA or 5-HIAA, but NEOP and BH4 were higher in RLS patients. The RLS group was significantly older than the control group (64.2 +/- 9.2 years vs. 51.4 +/- 6.3 years; P < 0.001). A multiple regression analysis showed a strong correlation between age and 5-HIAA (r = 0.46, P = 0.04) and between age and NEOP (r = 0.61, P < 0.01). To eliminate the potential error created by the age difference between groups, an age-adjusted subgroup of RLS and control subjects were compared. There was still no difference found for HVA; however, 5-HIAA was now significantly lower (P < 0.01) in the RLS subgroup. Age-adjustment eliminated the differences previously found for NEOP, (P = 0.12), but BH4 continued to remain higher in the RLS group (P < 0.01). CONCLUSION: Differences in CSFHVA concentrations were not found. The changes in 5-HIAA and BH4 are of unclear clinical significance and require further assessment with appropriate age-matched controls.
Authors: R Ye; A M D Carneiro; Q Han; D Airey; E Sanders-Bush; B Zhang; L Lu; R Williams; R D Blakely Journal: Genes Brain Behav Date: 2014-02-06 Impact factor: 3.449
Authors: Christopher J Earley; Hiroto Kuwabara; Dean F Wong; Charlene Gamaldo; Rachel Salas; James Brasic; Hayden T Ravert; Robert F Dannals; Richard P Allen Journal: Sleep Date: 2011-03-01 Impact factor: 5.849
Authors: Richard P Allen; Nathan C Donelson; Byron C Jones; Yuqing Li; Mauro Manconi; David B Rye; Subhabrata Sanyal; Juliane Winkelmann Journal: Sleep Med Date: 2016-09-02 Impact factor: 3.492
Authors: Shangru Lyu; Mark P DeAndrade; Stefan Mueller; Alexander Oksche; Arthur S Walters; Yuqing Li Journal: Behav Brain Res Date: 2019-07-31 Impact factor: 3.332
Authors: Shangru Lyu; Atbin Doroodchi; Hong Xing; Yi Sheng; Mark P DeAndrade; Youfeng Yang; Tracy L Johnson; Stefan Clemens; Fumiaki Yokoi; Michael A Miller; Rui Xiao; Yuqing Li Journal: Brain Struct Funct Date: 2020-05-28 Impact factor: 3.270