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Literature DB >> 11214751

Peptide and protein PEGylation: a review of problems and solutions.

Abstract

The paper discusses general problems in using PEG for conjugation to high or low molecular weight molecules. Methods of binding PEG to different functional groups in macromolecules is reported together with their eventual limitations. Problems encountered in conjugation, such as the evaluation of the number of PEG chains bound, the localisation of the site of conjugation in polypeptides and the procedure to direct PEGylation to the desired site in the molecule are discussed. Finally, the paper reports on more specific methods regarding reversible PEGylation, cross-linking reagents with PEG arms, PEG for enzyme solubilization in organic solvent and new polymers as alternative to PEG.

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Year: 2001 PMID： 11214751 DOI： 10.1016/s0142-9612(00)00193-9

Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479

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Cited

117 in total

1. Poly(zwitterionic)protein conjugates offer increased stability without sacrificing binding affinity or bioactivity.

Authors: Andrew J Keefe; Shaoyi Jiang
Journal: Nat Chem Date: 2011-12-11 Impact factor: 24.427

2. Sequence, structure, and function of peptide self-assembled monolayers.

Authors: Ann K Nowinski; Fang Sun; Andrew D White; Andrew J Keefe; Shaoyi Jiang
Journal: J Am Chem Soc Date: 2012-03-22 Impact factor: 15.419

Review 3. Structure-immunogenicity relationships of therapeutic proteins.

Authors: Suzanne Hermeling; Daan J A Crommelin; Huub Schellekens; Wim Jiskoot
Journal: Pharm Res Date: 2004-06 Impact factor: 4.200

4. Albumin-based nanoparticles as magnetic resonance contrast agents: II. Physicochemical characterisation of purified and standardised nanoparticles.

Authors: A A Abdelmoez; G C Thurner; E A Wallnöfer; N Klammsteiner; C Kremser; H Talasz; M Mrakovcic; E Fröhlich; W Jaschke; P Debbage
Journal: Histochem Cell Biol Date: 2010-07-14 Impact factor: 4.304

5. Direct quantitative analysis of a 20 kDa PEGylated human calcitonin gene peptide antagonist in cynomolgus monkey serum using in-source CID and UPLC-MS/MS.

Authors: Hongyan Li; Mark J Rose; Jerry Ryan Holder; Marie Wright; Les P Miranda; Christopher A James
Journal: J Am Soc Mass Spectrom Date: 2011-06-23 Impact factor: 3.109

Peptide and protein PEGylation: a review of problems and solutions.

1. Poly(zwitterionic)protein conjugates offer increased stability without sacrificing binding affinity or bioactivity.

2. Sequence, structure, and function of peptide self-assembled monolayers.

Review 3. Structure-immunogenicity relationships of therapeutic proteins.

4. Albumin-based nanoparticles as magnetic resonance contrast agents: II. Physicochemical characterisation of purified and standardised nanoparticles.

5. Direct quantitative analysis of a 20 kDa PEGylated human calcitonin gene peptide antagonist in cynomolgus monkey serum using in-source CID and UPLC-MS/MS.

Review 6. CNS drug delivery: opioid peptides and the blood-brain barrier.

Review 7. Nanomedicine: clinical applications of polyethylene glycol conjugated proteins and drugs.

8. A model of glycosylated human butyrylcholinesterase.

9. Synthesis, characterization and in vivo efficacy of PEGylated insulin for oral delivery with complexation hydrogels.

10. A highly efficient catalyst for oxime ligation and hydrazone-oxime exchange suitable for bioconjugation.