Literature DB >> 11210135

cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells.

S Ikawa1, Y Tsuda, T Fukada, K Sugimoto, M Himeno.   

Abstract

We cloned cDNA of three variants of BtR175, a putative Bombyx mori receptor for Bacillus thuringiensis Cry1Aa delta-endotoxin by PCR. These variants were likely to be allelic to BtR175. cDNA of BtR175b, the most distant variant from BtR175, was introduced into mammalian cells. BtR175b protein was expressed in the plasma membrane of the cells and showed binding activity to Cry1Aa.

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Year:  2000        PMID： 11210135     DOI： 10.1271/bbb.64.2682

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  4 in total

Review 1.  Role of receptors in Bacillus thuringiensis crystal toxin activity.

Authors:  Craig R Pigott; David J Ellar
Journal:  Microbiol Mol Biol Rev       Date:  2007-06       Impact factor: 11.056

2.  Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm).

Authors:  Yoko Tsuda; Fumiki Nakatani; Keiko Hashimoto; Satoshi Ikawa; Chikako Matsuura; Takashi Fukada; Kenji Sugimoto; Michio Himeno
Journal:  Biochem J       Date:  2003-02-01       Impact factor: 3.857

3.  A Spodoptera exigua cadherin serves as a putative receptor for Bacillus thuringiensis Cry1Ca toxin and shows differential enhancement of Cry1Ca and Cry1Ac toxicity.

Authors:  Xiang-Liang Ren; Rui-Rui Chen; Ying Zhang; Yan Ma; Jin-Jie Cui; Zhao-Jun Han; Li-Li Mu; Guo-Qing Li
Journal:  Appl Environ Microbiol       Date:  2013-07-08       Impact factor: 4.792

4.  Binding specificity of Bacillus thuringiensis Cry1Aa for purified, native Bombyx mori aminopeptidase N and cadherin-like receptors.

Authors:  J L Jenkins; D H Dean
Journal:  BMC Biochem       Date:  2001-10-16       Impact factor: 4.059
  4 in total

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