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Literature DB >> 11208812

Preferential cleavage of degradative intermediates of rpsT mRNA by the Escherichia coli RNA degradosome.

Abstract

RNase E, the principal RNase capable of initiating mRNA decay, preferentially attacks 5'-monophosphorylated over 5'-triphosphorylated substrates. Site-specific cleavage in vitro of the rpsT mRNA by RNase H directed by chimeric 2'-O-methyl oligonucleotides was employed to create truncated RNAs which are identical to authentic degradative intermediates. The rates of cleavage of two such intermediates by RNase E in the RNA degradosome are significantly faster (2.5- to 8-fold) than that of intact RNA. This verifies the preference of RNase E for degradative intermediates and can explain the frequent "all-or-none" behavior of mRNAs during the decay process.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2001 PMID： 11208812 PMCID： PMC94981 DOI： 10.1128/JB.183.3.1106-1109.2001

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

22 in total

1. Roles of polyadenylation and nucleolytic cleavage in the filamentous phage mRNA processing and decay pathways in Escherichia coli.

Authors: A F Goodrich; D A Steege
Journal: RNA Date: 1999-07 Impact factor: 4.942

Review 2. Emerging features of mRNA decay in bacteria.

Authors: D A Steege
Journal: RNA Date: 2000-08 Impact factor: 4.942

3. Secondary structure of the mRNA for ribosomal protein S20. Implications for cleavage by ribonuclease E.

Authors: G A Mackie
Journal: J Biol Chem Date: 1992-01-15 Impact factor: 5.157

4. Structural requirements for the processing of Escherichia coli 5 S ribosomal RNA by RNase E in vitro.

Authors: R S Cormack; G A Mackie
Journal: J Mol Biol Date: 1992-12-20 Impact factor: 5.469

5. Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro.

Authors: G A Mackie
Journal: J Bacteriol Date: 1991-04 Impact factor: 3.490

6. The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo.

Authors: S Lin-Chao; S N Cohen
Journal: Cell Date: 1991-06-28 Impact factor: 41.582

7. The role of RNA structure in determining RNase E-dependent cleavage sites in the mRNA for ribosomal protein S20 in vitro.

Authors: G A Mackie; J L Genereaux
Journal: J Mol Biol Date: 1993-12-20 Impact factor: 5.469

8. Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli.

Authors: B K Ghora; D Apirion
Journal: Cell Date: 1978-11 Impact factor: 41.582

9. Degradation of RNA in Escherichia coli. A hypothesis.

Authors: D Apirion
Journal: Mol Gen Genet Date: 1973-05-28

10. The CafA protein required for the 5'-maturation of 16 S rRNA is a 5'-end-dependent ribonuclease that has context-dependent broad sequence specificity.

Authors: M R Tock; A P Walsh; G Carroll; K J McDowall
Journal: J Biol Chem Date: 2000-03-24 Impact factor: 5.157

11 in total

Preferential cleavage of degradative intermediates of rpsT mRNA by the Escherichia coli RNA degradosome.

1. Roles of polyadenylation and nucleolytic cleavage in the filamentous phage mRNA processing and decay pathways in Escherichia coli.

Review 2. Emerging features of mRNA decay in bacteria.

3. Secondary structure of the mRNA for ribosomal protein S20. Implications for cleavage by ribonuclease E.

4. Structural requirements for the processing of Escherichia coli 5 S ribosomal RNA by RNase E in vitro.

5. Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro.

6. The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo.

7. The role of RNA structure in determining RNase E-dependent cleavage sites in the mRNA for ribosomal protein S20 in vitro.

8. Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli.

9. Degradation of RNA in Escherichia coli. A hypothesis.

10. The CafA protein required for the 5'-maturation of 16 S rRNA is a 5'-end-dependent ribonuclease that has context-dependent broad sequence specificity.

Review 1. mRNA decay in Escherichia coli comes of age.

Review 2. Processing endoribonucleases and mRNA degradation in bacteria.

3. Distinct Requirements for 5'-Monophosphate-assisted RNA Cleavage by Escherichia coli RNase E and RNase G.

4. Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal.

5. Substrate binding and active site residues in RNases E and G: role of the 5'-sensor.

6. The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection.

7. Identification of the RNA Pyrophosphohydrolase RppH of Helicobacter pylori and Global Analysis of Its RNA Targets.

8. Initiation of decay of Bacillus subtilis rpsO mRNA by endoribonuclease RNase Y.

9. Influence of translation on RppH-dependent mRNA degradation in Escherichia coli.

10. Post-transcriptional processing of the LEE4 operon in enterohaemorrhagic Escherichia coli.