UNLABELLED: The aim of this study was to establish the accuracy of determination of fibrinolytic proteins in human bile using commercial kits. MATERIAL AND METHOD: Gall bladder bile was obtained from patient undergoing laparoscopic cholecystectomy. The concentrations of tissue plasminogen activator (tPA), urokinase (uPA) and plasminogen activator inhibitor-1 (PAI-1) and inhibitor-2 (PAI-2) in bile were measured by enzyme linked immunoassay. Afterwards we conducted so called 'recovery test'. RESULTS: Recovery percents of t-PA, u-PA, PAI-1, PAI 2 were very high i.e. 91.94, 94.07, 93.95, 91.2. We concluded that human fibrinolytic proteins in bile could be detected very accurately using commercial kits.
UNLABELLED: The aim of this study was to establish the accuracy of determination of fibrinolytic proteins in human bile using commercial kits. MATERIAL AND METHOD: Gall bladder bile was obtained from patient undergoing laparoscopic cholecystectomy. The concentrations of tissue plasminogen activator (tPA), urokinase (uPA) and plasminogen activator inhibitor-1 (PAI-1) and inhibitor-2 (PAI-2) in bile were measured by enzyme linked immunoassay. Afterwards we conducted so called 'recovery test'. RESULTS: Recovery percents of t-PA, u-PA, PAI-1, PAI 2 were very high i.e. 91.94, 94.07, 93.95, 91.2. We concluded that human fibrinolytic proteins in bile could be detected very accurately using commercial kits.
Authors: Troy J Kemp; Felipe A Castro; Yu-Tang Gao; Allan Hildesheim; Leticia Nogueira; Bing-Sheng Wang; Lu Sun; Gloriana Shelton; Ruth M Pfeiffer; Ann W Hsing; Ligia A Pinto; Jill Koshiol Journal: Cytokine Date: 2015-03-02 Impact factor: 3.861