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Literature DB >> 11206007

Altered nuclear localization of bax protein in BCNU-resistant glioma cells.

Abstract

To investigate the role of apoptosis suppression in glioma chemotherapy resistance, protein levels and subcellular localization of bcl-2 family members were investigated in 2 pairs of sensitive cell lines and their in vitro generated resistant derivatives. The alkylating agent, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), induced apoptosis in both sensitive cell strains and apoptosis was suppressed in both resistant derivatives. Both resistant cell lines contained altered regulation of a bcl-2 related protein consistent with the suppression of apoptosis. Independent of which bcl-2 family member was dysregulated, resistance was associated with altered regulation in the subcellular localization of bax protein. Following BCNU treatment, bax accumulated in nucleoli and a nuclei containing fraction of sensitive cells but not their resistant derivatives. Nuclear accumulation was an early event in apoptosis induction. These data indicates altered subcellular localization of bax may play a role in resistance. In addition, the association between an early, nucleolar localization of bax and the induction of apoptosis suggests that localization of bax to nucleoli may play a role in apoptosis-induction of glioma cells.

Entities: Chemical Disease Gene

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Year: 2000 PMID： 11206007 DOI： 10.1023/a:1026574123273

Source DB: PubMed Journal: J Neurooncol ISSN： 0167-594X Impact factor: 4.130

23 in total

1. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis.

Authors: Y T Hsu; K G Wolter; R J Youle
Journal: Proc Natl Acad Sci U S A Date: 1997-04-15 Impact factor: 11.205

2. Isolation, karyotype, and clonal growth of heterogeneous subpopulations of human malignant gliomas.

Authors: J R Shapiro; W K Yung; W R Shapiro
Journal: Cancer Res Date: 1981-06 Impact factor: 12.701

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Authors: S Desnoyers; S H Kaufmann; G G Poirier
Journal: Exp Cell Res Date: 1996-08-25 Impact factor: 3.905

4. Basic fibroblast growth factor enters the nucleolus and stimulates the transcription of ribosomal genes in ABAE cells undergoing G0----G1 transition.

Authors: G Bouche; N Gas; H Prats; V Baldin; J P Tauber; J Teissié; F Amalric
Journal: Proc Natl Acad Sci U S A Date: 1987-10 Impact factor: 11.205

5. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2.

Authors: S Krajewski; M Krajewska; A Shabaik; T Miyashita; H G Wang; J C Reed
Journal: Am J Pathol Date: 1994-12 Impact factor: 4.307

6. Exclusion of Int-6 from PML nuclear bodies by binding to the HTLV-I Tax oncoprotein.

Authors: C Desbois; R Rousset; F Bantignies; P Jalinot
Journal: Science Date: 1996-08-16 Impact factor: 47.728

7. Nucleolar localization of parathyroid hormone-related peptide enhances survival of chondrocytes under conditions that promote apoptotic cell death.

Authors: J E Henderson; N Amizuka; H Warshawsky; D Biasotto; B M Lanske; D Goltzman; A C Karaplis
Journal: Mol Cell Biol Date: 1995-08 Impact factor: 4.272

8. Translocation of retinoblastoma protein associated with tumor cell growth inhibition.

Authors: V Rogalsky; G Todorov; D Moran
Journal: Biochem Biophys Res Commun Date: 1993-05-14 Impact factor: 3.575

9. Movement of Bax from the cytosol to mitochondria during apoptosis.

Authors: K G Wolter; Y T Hsu; C L Smith; A Nechushtan; X G Xi; R J Youle
Journal: J Cell Biol Date: 1997-12-01 Impact factor: 10.539

10. A stress-inducible 40 kDa protein (hsp40): purification by modified two-dimensional gel electrophoresis and co-localization with hsc70(p73) in heat-shocked HeLa cells.

Authors: H Hattori; T Kaneda; B Lokeshwar; A Laszlo; K Ohtsuka
Journal: J Cell Sci Date: 1993-03 Impact factor: 5.285

6 in total

Altered nuclear localization of bax protein in BCNU-resistant glioma cells.

1. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis.

2. Isolation, karyotype, and clonal growth of heterogeneous subpopulations of human malignant gliomas.

3. Alteration of the nucleolar localization of poly(ADP-ribose) polymerase upon treatment with transcription inhibitors.

4. Basic fibroblast growth factor enters the nucleolus and stimulates the transcription of ribosomal genes in ABAE cells undergoing G0----G1 transition.

5. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2.

6. Exclusion of Int-6 from PML nuclear bodies by binding to the HTLV-I Tax oncoprotein.

7. Nucleolar localization of parathyroid hormone-related peptide enhances survival of chondrocytes under conditions that promote apoptotic cell death.

8. Translocation of retinoblastoma protein associated with tumor cell growth inhibition.

9. Movement of Bax from the cytosol to mitochondria during apoptosis.

10. A stress-inducible 40 kDa protein (hsp40): purification by modified two-dimensional gel electrophoresis and co-localization with hsc70(p73) in heat-shocked HeLa cells.

1. Proton and sodium MRI assessment of emerging tumor chemotherapeutic resistance.

2. Sodium magnetic resonance imaging of chemotherapeutic response in a rat glioma.

3. Expression profile of the proapoptotic protein Bax in the human brain.

4. Bim nuclear translocation and inactivation by viral interferon regulatory factor.

Review 5. Pharmacotherapy for adults with tumors of the central nervous system.

6. Identification of nucleolus localization signal of betanodavirus GGNNV protein alpha.