Literature DB >> 11202228

Immunofluorescence analysis of T-cell responses in health and disease.

H T Maecker1, V C Maino, L J Picker.   

Abstract

The use of flow cytometry to study the functional responses of T cells by immunofluorescent staining for intracellular cytokines and other markers is a growing field of clinical interest. In this article, we describe methods for the rapid evaluation of T-cell responses to mitogens and specific antigens and explore how these assays might be valuable in various clinical settings.

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Year:  2000        PMID： 11202228     DOI： 10.1023/a:1026403724413

Source DB:  PubMed          Journal:  J Clin Immunol        ISSN： 0271-9142            Impact factor:   8.317


  46 in total

1.  The generation and maintenance of memory T and B cells.

Authors:  R W Dutton; S L Swain; L M Bradley
Journal:  Immunol Today       Date:  1999-07

Review 2.  Measurement of anti-human cytomegalovirus T cell reactivity in transplant recipients and its potential clinical use: a mini-review.

Authors:  F Kern; N Faulhaber; E Khatamzas; C Frömmel; R Ewert; S Prösch; H Volk; P Reinke
Journal:  Intervirology       Date:  1999       Impact factor: 1.763

Review 3.  The CD4(+) T cell response to HIV-1.

Authors:  L J Picker; V C Maino
Journal:  Curr Opin Immunol       Date:  2000-08       Impact factor: 7.486

4.  T-cell epitope mapping by flow cytometry.

Authors:  F Kern; I P Surel; C Brock; B Freistedt; H Radtke; A Scheffold; R Blasczyk; P Reinke; J Schneider-Mergener; A Radbruch; P Walden; H D Volk
Journal:  Nat Med       Date:  1998-08       Impact factor: 53.440

5.  Levels of human immunodeficiency virus type 1-specific cytotoxic T-lymphocyte effector and memory responses decline after suppression of viremia with highly active antiretroviral therapy.

Authors:  S A Kalams; P J Goulder; A K Shea; N G Jones; A K Trocha; G S Ogg; B D Walker
Journal:  J Virol       Date:  1999-08       Impact factor: 5.103

6.  Optimization of whole blood antigen-specific cytokine assays for CD4(+) T cells.

Authors:  L E Nomura; J M Walker; H T Maecker
Journal:  Cytometry       Date:  2000-05-01

7.  Anti-human immunodeficiency virus type 1 (HIV-1) CD8(+) T-lymphocyte reactivity during combination antiretroviral therapy in HIV-1-infected patients with advanced immunodeficiency.

Authors:  C R Rinaldo; X L Huang; Z Fan; J B Margolick; L Borowski; A Hoji; C Kalinyak; D K McMahon; S A Riddler; W H Hildebrand; R B Day; J W Mellors
Journal:  J Virol       Date:  2000-05       Impact factor: 5.103

8.  Upregulation of Fas ligand expression by human immunodeficiency virus in human macrophages mediates apoptosis of uninfected T lymphocytes.

Authors:  A D Badley; J A McElhinny; P J Leibson; D H Lynch; M R Alderson; C V Paya
Journal:  J Virol       Date:  1996-01       Impact factor: 5.103

9.  Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.

Authors:  B Sander; I Höidén; U Andersson; E Möller; J S Abrams
Journal:  J Immunol Methods       Date:  1993-12-03       Impact factor: 2.303

10.  Viral immune evasion due to persistence of activated T cells without effector function.

Authors:  A J Zajac; J N Blattman; K Murali-Krishna; D J Sourdive; M Suresh; J D Altman; R Ahmed
Journal:  J Exp Med       Date:  1998-12-21       Impact factor: 14.307
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  14 in total

1.  An anti-CD45RO immunotoxin kills HIV-latently infected cells from individuals on HAART with little effect on CD8 memory.

Authors:  J Saavedra-Lozano; Y Cao; J Callison; R Sarode; D Sodora; J Edgar; J Hatfield; L Picker; D Peterson; O Ramilo; E S Vitetta
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-24       Impact factor: 11.205

2.  Effect on cellular and humoral immune responses of the AS03 adjuvant system in an A/H1N1/2009 influenza virus vaccine administered to adults during two randomized controlled trials.

Authors:  François Roman; Frédéric Clément; Walthère Dewé; Karl Walravens; Cathy Maes; Julie Willekens; Fien De Boever; Emmanuel Hanon; Geert Leroux-Roels
Journal:  Clin Vaccine Immunol       Date:  2011-03-30

3.  Evaluation of the safety and immunogenicity of two antigen concentrations of the Mtb72F/AS02(A) candidate tuberculosis vaccine in purified protein derivative-negative adults.

Authors:  Isabel Leroux-Roels; Geert Leroux-Roels; Opokua Ofori-Anyinam; Philippe Moris; Els De Kock; Frédéric Clement; Marie-Claude Dubois; Marguerite Koutsoukos; Marie-Ange Demoitié; Joe Cohen; W Ripley Ballou
Journal:  Clin Vaccine Immunol       Date:  2010-09-22

4.  Correlate of immune protection against HSV-1 genital disease in vaccinated women.

Authors:  Robert B Belshe; Thomas C Heineman; David I Bernstein; Abbie R Bellamy; Marian Ewell; Robbert van der Most; Carolyn D Deal
Journal:  J Infect Dis       Date:  2013-11-27       Impact factor: 5.226

5.  Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot Assay but Not CMV QuantiFERON Assay Is a Novel Biomarker To Determine Risk of Congenital CMV Infection in Pregnant Women.

Authors:  Gabriella Forner; Alda Saldan; Carlo Mengoli; Nadia Gussetti; Giorgio Palù; Davide Abate
Journal:  J Clin Microbiol       Date:  2016-06-08       Impact factor: 5.948

Review 6.  Beyond 51Cr release: New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues.

Authors:  B L Shacklett
Journal:  Clin Exp Immunol       Date:  2002-11       Impact factor: 4.330

7.  T cell responses to the RTS,S/AS01(E) and RTS,S/AS02(D) malaria candidate vaccines administered according to different schedules to Ghanaian children.

Authors:  Daniel Ansong; Kwaku P Asante; Johan Vekemans; Sandra K Owusu; Ruth Owusu; Naana A W Brobby; David Dosoo; Alex Osei-Akoto; Kingsley Osei-Kwakye; Emmanuel Asafo-Adjei; Kwadwo O Boahen; Justice Sylverken; George Adjei; David Sambian; Stephen Apanga; Kingsley Kayan; Michel H Janssens; Marc J J Lievens; Aurelie C Olivier; Erik Jongert; Patrice Dubois; Barbara M Savarese; Joe Cohen; Sampson Antwi; Brian M Greenwood; Jennifer A Evans; Tsiri Agbenyega; Philippe J Moris; Seth Owusu-Agyei
Journal:  PLoS One       Date:  2011-04-27       Impact factor: 3.240

8.  Immunogenicity profile of a 3.75-μg hemagglutinin pandemic rH5N1 split virion AS03A-adjuvanted vaccine in elderly persons: a randomized trial.

Authors:  Stéphane Heijmans; Marc De Meulemeester; Paul Reynders; Didier Giet; Etienne Demanet; Pierre-Yves Devresse; Giancarlo Icardi; Mamadou Dramé; François Roman; Paul Gillard
Journal:  J Infect Dis       Date:  2011-04-15       Impact factor: 5.226

9.  Performance of plate-based cytokine flow cytometry with automated data analysis.

Authors:  Maria A Suni; Holli S Dunn; Patricia L Orr; Rian de Laat; Elizabeth Sinclair; Smita A Ghanekar; Barry M Bredt; John F Dunne; Vernon C Maino; Holden T Maecker
Journal:  BMC Immunol       Date:  2003-09-02       Impact factor: 3.615

10.  Long-term booster schedules with AS03A-adjuvanted heterologous H5N1 vaccines induces rapid and broad immune responses in Asian adults.

Authors:  Paul Gillard; Daniel Wai Sing Chu; Shinn-Jang Hwang; Pan-Chyr Yang; Prasert Thongcharoen; Fong Seng Lim; Mamadou Dramé; Karl Walravens; François Roman
Journal:  BMC Infect Dis       Date:  2014-03-15       Impact factor: 3.090
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