A novel PCR-RFLP assay for the detection of a polymorphism in the 3' of STAT6 gene.

We have identified a novel single nucleotide polymorphism at position 2964 (G/A) in the 3' untranslated region of the human STAT6 gene (GeneBank Accession No. U16031). A novel PCR-RFLP method has been devised for this polymorphism using the amplification-created restriction site (ACRS) method. Allele frequencies in the UK Caucasian population were found to be 0.33 for allele A and 0.67 for allele G.