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Literature DB >> 11196570

Nonenzymatic template-directed condensation of short-chained oligouridylates on a poly(A) template.

Abstract

An oligouridylate with chain-length of more than three was condensed on a polyadenylate [poly(A)] template with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) in imidazole buffer. The condensation reaction proceeds via the phosphorimidazolide of the oligouridylate as an intermediate. Pentauridylate [(pU)5] was converted to decauridylate [(pU)10] in 10% yield at 0 degree C for 7 days in the presence of the poly(A) template, while no coupling product was obtained in the absence of the poly(A) template. The resulting linkage of the (pU)10 was mainly 2'-5' linkage.

Entities: Chemical

Mesh：

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Year: 2000 PMID： 11196570 DOI： 10.1023/a:1026583814064

Source DB: PubMed Journal: Orig Life Evol Biosph ISSN： 0169-6149 Impact factor: 1.950

21 in total

1. Template-directed synthesis of oligonucleotides under eutectic conditions.

Authors: R Stribling; S L Miller
Journal: J Mol Evol Date: 1991 Impact factor: 2.395

2. Unnatural selection in chemical systems.

Authors: L E Orgel
Journal: Acc Chem Res Date: 1995-03 Impact factor: 22.384

Review 3. RNA catalysis and the origins of life.

Authors: L E Orgel
Journal: J Theor Biol Date: 1986-11-21 Impact factor: 2.691

4. Non-enzymic oligonucleotide synthesis on a polycytidylate template.

Authors: J Sulston; R Lohrmann; L E Orgel; H Schneider-Bernloehr; B J Weimann; H T Miles
Journal: J Mol Biol Date: 1969-03-14 Impact factor: 5.469

5. Studies on some interactions and reactions of oligonucleotides in aqueous solution.

Authors: R Naylor; P T Gilham
Journal: Biochemistry Date: 1966-08 Impact factor: 3.162

6. Conformational and stacking properties of 3'-5' and 2'-5' linked oligoribonucleotides studied by CD.

Authors: H Sawai; K Kuroda; J Seki; H Ozaki
Journal: Biopolymers Date: 1996-08 Impact factor: 2.505

7. Helical structure formation between complementary oligonucleotides. Minimum chain length required for the template-directed synthesis of oligonucleotides.

Authors: H Sawai; S Totuka; K Yamamoto
Journal: Orig Life Evol Biosph Date: 1997-12 Impact factor: 1.950

8. Attempted nonenzymatic template-directed oligomerizations on a polyadenylic acid template: implications for the nature of the first genetic material.

Authors: R Stribling; S L Miller
Journal: J Mol Evol Date: 1991 Impact factor: 2.395

Review 4. Taming Prebiotic Chemistry: The Role of Heterogeneous and Interfacial Catalysis in the Emergence of a Prebiotic Catalytic/Information Polymer System.

Authors: Pierre-Alain Monnard
Journal: Life (Basel) Date: 2016-11-04

4 in total

Nonenzymatic template-directed condensation of short-chained oligouridylates on a poly(A) template.

1. Template-directed synthesis of oligonucleotides under eutectic conditions.

2. Unnatural selection in chemical systems.

Review 3. RNA catalysis and the origins of life.

4. Non-enzymic oligonucleotide synthesis on a polycytidylate template.

5. Studies on some interactions and reactions of oligonucleotides in aqueous solution.

6. Conformational and stacking properties of 3'-5' and 2'-5' linked oligoribonucleotides studied by CD.

7. Helical structure formation between complementary oligonucleotides. Minimum chain length required for the template-directed synthesis of oligonucleotides.

8. Attempted nonenzymatic template-directed oligomerizations on a polyadenylic acid template: implications for the nature of the first genetic material.

9. Heteropolynucleotides as templates for non-enzymatic polymerizations.

10. A nonenzymatic RNA polymerase model.

1. Ligation of the hairpin ribozyme in cis induced by freezing and dehydration.

2. Prebiotically plausible mechanisms increase compositional diversity of nucleic acid sequences.

3. Enzyme-free ligation of dimers and trimers to RNA primers.

Review 4. Taming Prebiotic Chemistry: The Role of Heterogeneous and Interfacial Catalysis in the Emergence of a Prebiotic Catalytic/Information Polymer System.