Literature DB >> 11196321

System for cleavable Fc fusion proteins using tobacco etch virus (TEV) protease.

J Haspel1, C Blanco, J Jacob, M Grumet.   

Abstract

We describe a novel Fc fusion protein system that can be cleaved by tobacco etch virus (TEV) protease. This system is desirable because it takes advantage of the high specificity of TEV protease and its activity at 4 degrees C. We produced two TEV-Fc fusion proteins that contain the first three Ig domains and all six Ig domains of the cell adhesion molecule L1. Both proteins were efficiently cleaved by TEV protease at 4 degrees C. Functional analysis of the cleavage products in neurite outgrowth assays showed they had similar activities to their parental Fc fusion proteins. Therefore, TEV-Fc fusion proteins may increase the utility and flexibility of the Fc fusion protein system.

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Year:  2001        PMID： 11196321     DOI： 10.2144/01301st01

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  10 in total

1.  Cell adhesion molecule L1 in folded (horseshoe) and extended conformations.

Authors:  G Schürmann; J Haspel; M Grumet; H P Erickson
Journal:  Mol Biol Cell       Date:  2001-06       Impact factor: 4.138

2.  Targeting of adenovirus via genetic modification of the viral capsid combined with a protein bridge.

Authors:  Nikolay Korokhov; Galina Mikheeva; Alexander Krendelshchikov; Natalya Belousova; Vera Simonenko; Valentina Krendelshchikova; Alexander Pereboev; Alexander Kotov; Olga Kotova; Pierre L Triozzi; Wayne A Aldrich; Joanne T Douglas; Kin-Ming Lo; Papia T Banerjee; Stephen D Gillies; David T Curiel; Victor Krasnykh
Journal:  J Virol       Date:  2003-12       Impact factor: 5.103

3.  Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1.

Authors:  Nikolaj Kulahin; Christina Kasper; Ole Kristensen; Jette Sandholm Kastrup; Vladimir Berezin; Elisabeth Bock; Michael Gajhede
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-08-31

4.  Mitogen-activated protein kinase modulates ethanol inhibition of cell adhesion mediated by the L1 neural cell adhesion molecule.

Authors:  Xiaowei Dou; Michael F Wilkemeyer; Carrie E Menkari; Scott E Parnell; Kathleen K Sulik; Michael E Charness
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-19       Impact factor: 11.205

5.  A hingeless Fc fusion system for site-specific cleavage by IdeS.

Authors:  Shabazz Novarra; Luba Grinberg; Keith W Rickert; Arnita Barnes; Susan Wilson; Manuel Baca
Journal:  MAbs       Date:  2016-05-21       Impact factor: 5.857

6.  Structural characterization of the active site of the PduO-type ATP:Co(I)rrinoid adenosyltransferase from Lactobacillus reuteri.

Authors:  Martin St Maurice; Paola E Mera; María P Taranto; Fernando Sesma; Jorge C Escalante-Semerena; Ivan Rayment
Journal:  J Biol Chem       Date:  2006-11-22       Impact factor: 5.157

7.  Dihydroflavin-driven adenosylation of 4-coordinate Co(II) corrinoids: are cobalamin reductases enzymes or electron transfer proteins?

Authors:  Paola E Mera; Jorge C Escalante-Semerena
Journal:  J Biol Chem       Date:  2009-11-21       Impact factor: 5.157

8.  Cadherin-8 and N-cadherin differentially regulate pre- and postsynaptic development of the hippocampal mossy fiber pathway.

Authors:  Iddil H Bekirov; Vanja Nagy; Alexandra Svoronos; George W Huntley; Deanna L Benson
Journal:  Hippocampus       Date:  2008       Impact factor: 3.899

9.  Ethanol disrupts axon outgrowth stimulated by netrin-1, GDNF, and L1 by blocking their convergent activation of Src family kinase signaling.

Authors:  Suzhen Chen; Michael E Charness
Journal:  J Neurochem       Date:  2012-09-28       Impact factor: 5.372

10.  Structure of the Archaeoglobus fulgidus orphan ORF AF1382 determined by sulfur SAD from a moderately diffracting crystal.

Authors:  Jin-Yi Zhu; Zheng-Qing Fu; Lirong Chen; Hao Xu; John Chrzas; John Rose; Bi-Cheng Wang
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2012-08-18
  10 in total

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