OBJECTIVE: To compare the diagnostic performance of a complement fixation test, an agar gel immunodiffusion test, an enzyme-linked immunosorbent assay, and a whole-blood interferon-gamma assay for paratuberculosis in 14 sheep experimentally infected with Mycobacterium avium subsp paratuberculosis. EXPERIMENTAL DESIGN: Longitudinal study. RESULTS: The IFN-gamma assay detected more experimentally infected sheep, and earlier, than any of the serological tests. None of the antibody assays was able to detect all sheep with histologically confirmed paratuberculosis. CONCLUSIONS: The superior performance of the IFN-gamma assay in detecting infected sheep in this small experimental population warrants its further evaluation in a larger population of sheep naturally exposed to M a paratuberculosis.
OBJECTIVE: To compare the diagnostic performance of a complement fixation test, an agar gel immunodiffusion test, an enzyme-linked immunosorbent assay, and a whole-blood interferon-gamma assay for paratuberculosis in 14 sheep experimentally infected with Mycobacterium avium subsp paratuberculosis. EXPERIMENTAL DESIGN: Longitudinal study. RESULTS: The IFN-gamma assay detected more experimentally infected sheep, and earlier, than any of the serological tests. None of the antibody assays was able to detect all sheep with histologically confirmed paratuberculosis. CONCLUSIONS: The superior performance of the IFN-gamma assay in detecting infected sheep in this small experimental population warrants its further evaluation in a larger population of sheep naturally exposed to M a paratuberculosis.
Authors: Ratna B Gurung; Douglas J Begg; Auriol C Purdie; Kumudika de Silva; John P Bannantine; Richard J Whittington Journal: Clin Vaccine Immunol Date: 2014-04-02
Authors: James W Wynne; Brian J Shiell; Michelle L Colgrave; Jill A Vaughan; Gary Beddome; Wojtek P Michalski Journal: Proteome Sci Date: 2012-03-26 Impact factor: 2.480
Authors: Randal T Capsel; Charles O Thoen; Timothy A Reinhardt; John D Lippolis; Renee Olsen; Judith R Stabel; John P Bannantine Journal: PLoS One Date: 2016-05-02 Impact factor: 3.240