S M Huang1, N M Strangman, J M Walker. 1. Alan M Schrier Research Laboratory, Departments of Psychology and Neuroscience, Brown University, Providence, RI 02912, USA.
Abstract
AIM: To develop a sensitive method for measuring the putative endocannabinoid 2-arachidonylglycerol (2-AG) in the peripheral and central nervous system. METHODS: A method using atmospheric pressure chemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS) was developed to determine the levels of 2-AG in methanol extracts of the rat lumbar spinal cord, dorsal root ganglion (DRG), and sciatic nerve. RESULTS: 2-AG was detected with high sensitivity and minimal sample preparation. The levels in the tissues analyzed were < or = pmol/mg wet weight. Similar levels were found in the spinal cord and the DRG, whereas approximately 7-fold lower levels were observed in the sciatic nerve. CONCLUSION: 2-AG is present in the peripheral nervous system, and the levels are markedly higher in cell bodies than those in axons.
AIM: To develop a sensitive method for measuring the putative endocannabinoid2-arachidonylglycerol (2-AG) in the peripheral and central nervous system. METHODS: A method using atmospheric pressure chemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS) was developed to determine the levels of 2-AG in methanol extracts of the rat lumbar spinal cord, dorsal root ganglion (DRG), and sciatic nerve. RESULTS:2-AG was detected with high sensitivity and minimal sample preparation. The levels in the tissues analyzed were < or = pmol/mg wet weight. Similar levels were found in the spinal cord and the DRG, whereas approximately 7-fold lower levels were observed in the sciatic nerve. CONCLUSION:2-AG is present in the peripheral nervous system, and the levels are markedly higher in cell bodies than those in axons.
Authors: Amy Wong; Devi R Sagar; Catharine A Ortori; David A Kendall; Victoria Chapman; David A Barrett Journal: J Lipid Res Date: 2014-07-25 Impact factor: 5.922