Targeting regulatory factors to intranuclear replication sites.

Plenty of evidence exists that mammalian nuclei are highly organized. Complex biochemical processes like DNA replication take place at specialized subnuclear sites and proteins directly or indirectly involved are concentrated at these sites. DNA replication is being used as a paradigm to study this functional organization of the nucleus, its underlying principles, and its potential regulatory consequences. In this review we discuss which factors were shown to be localized at nuclear replication sites, how they get there, and what role this might play in the precise, genome-wide regulation and coordination of complex biochemical processes.