| Literature DB >> 11177559 |
N A Habib1, C E Sarraf, R R Mitry, R Havlík, J Nicholls, M Kelly, C C Vernon, D Gueret-Wardle, R El-Masry, H Salama, R Ahmed, N Michail, E Edward, S L Jensen.
Abstract
Clinical studies were performed with a recombinant mutant adenovirus with an E1B 55-kDa deletion, dl1520, to assess its toxicity and efficacy in patients with irresectable primary and secondary liver tumors. A phase I study showed that dl1520 was well tolerated when administered directly intratumorally, intraarterially, or intravenously up to a dose of 3 x 10(11) PFU. Ultrastructural examination of tissue showed the presence of adenovirus in cell cytoplasm around the nucleus and revealed two dissimilar end points of cell death after virus infection: a preapoptotic sequence and necrosis. A phase II study showed that the combination of dl1520 and 5-fluorouracil (5-FU), when infused into the hepatic artery, was well tolerated. Further improvement in the recombinant vector design will be needed in order to achieve better clinical response.Entities:
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Year: 2001 PMID： 11177559 DOI： 10.1089/10430340150218369
Source DB: PubMed Journal: Hum Gene Ther ISSN： 1043-0342 Impact factor: 5.695