S R Chavali1, T Utsunomiya, R A Forse. 1. Department of Surgery, Harvard Institutes of Medicine, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, MA, USA.
Abstract
OBJECTIVES: Lignans that present in the nonfat portion of sesame seed oil (SSO) can inhibit delta-5 desaturase activity, resulting in an increase in the accumulation of dihomo-gamma-linolenic acid and, subsequently, decrease the production of proinflammatory dienoic eicosanoids with a concomitant increase in the secretion of less inflammatory monoenoic eicosanoids. DESIGN: Female Balb/c mice were fed diets supplemented with 5wt% SSO or a physical mixture of oils (control) whose fatty acid composition resembled that of SSO for 3 wks. MEASUREMENTS AND MAIN RESULTS: During a 4-day observation period after cecal ligation and puncture, only 20% of the controls and as many as 65% in the SSO group survived. Furthermore, the levels of cytokines and dienoic eicosanoids produced in response to an intraperitoneal injection of a nonlethal dose (50 microg/mouse) of endotoxin were measured in both groups. The interleukin (IL)-10 levels were markedly higher in mice fed SSO diets compared with the controls. However, the plasma concentrations of prostaglandin E1 + 2, tumor necrosis factor-alpha, IL-6, and IL-12 did not differ significantly between the two groups of mice. CONCLUSIONS: Because the fatty acid composition is almost similar between the two diets, sesamin, sesamol and other lignans in SSO appear to be responsible for an increase in survival after cecal ligation and puncture and also for an increase in the IL-10 levels in response to a nonlethal dose of endotoxin in mice.
OBJECTIVES:Lignans that present in the nonfat portion of sesame seed oil (SSO) can inhibit delta-5 desaturase activity, resulting in an increase in the accumulation of dihomo-gamma-linolenic acid and, subsequently, decrease the production of proinflammatory dienoiceicosanoids with a concomitant increase in the secretion of less inflammatory monoenoic eicosanoids. DESIGN: Female Balb/c mice were fed diets supplemented with 5wt% SSO or a physical mixture of oils (control) whose fatty acid composition resembled that of SSO for 3 wks. MEASUREMENTS AND MAIN RESULTS: During a 4-day observation period after cecal ligation and puncture, only 20% of the controls and as many as 65% in the SSO group survived. Furthermore, the levels of cytokines and dienoiceicosanoids produced in response to an intraperitoneal injection of a nonlethal dose (50 microg/mouse) of endotoxin were measured in both groups. The interleukin (IL)-10 levels were markedly higher in mice fed SSO diets compared with the controls. However, the plasma concentrations of prostaglandin E1 + 2, tumor necrosis factor-alpha, IL-6, and IL-12 did not differ significantly between the two groups of mice. CONCLUSIONS: Because the fatty acid composition is almost similar between the two diets, sesamin, sesamol and other lignans in SSO appear to be responsible for an increase in survival after cecal ligation and puncture and also for an increase in the IL-10 levels in response to a nonlethal dose of endotoxin in mice.