| Literature DB >> 11172719 |
H Shiratori1, R Sakuma, M Watanabe, H Hashiguchi, K Mochida, Y Sakai, J Nishino, Y Saijoh, M Whitman, H Hamada.
Abstract
Pitx2 is left--right (L--R) asymmetrically expressed initially in the lateral plate and later in primordial visceral organs. The transcriptional regulatory mechanisms that underlie L--R asymmetric expression of Pitx2 were investigated. Mouse Pitx2 has a left side-specific enhancer (ASE) that mediates both the initiation and maintenance of L--R asymmetric expression. This element contains three binding sites for the transcription factor FAST. The FAST binding sites function as Nodal-responsive elements and are sufficient for the initiation but not for the maintenance of asymmetric expression. The maintenance requires an Nkx2-5 binding site also present within the ASE. These results suggest that the left-sided expression of Pitx2 is directly initiated by Nodal signaling and is subsequently maintained by Nkx2. Such two-step control may represent a general mechanism for gene regulation during development.Entities:
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Year: 2001 PMID： 11172719 DOI： 10.1016/s1097-2765(01)00162-9
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970