Literature DB >> 11170556

Colorimetric method for the determination of lipoxygenase activity.

G E Anthon1, D M Barrett.   

Abstract

A colorimetic assay for lipoxygenase activity has been developed. The assay is based on the detection of the lipoxygenase reaction product, linoleic acid hydroperoxide, by the oxidative coupling of 3-methyl-2-benzothiazolinone (MBTH) with 3-(dimethylamino)benzoic acid (DMAB) in a hemoglobin-catalyzed reaction. This test reaction is rapid and sensitive, and it offers advantages over other methods for detecting lipoxygenase activity. The assay is capable of detecting activity in a number of crude vegetable homogenates and should be particularly useful where a rapid visual determination of lipoxygenase activity is desired.

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Year:  2001        PMID： 11170556     DOI： 10.1021/jf000871s

Source DB:  PubMed          Journal:  J Agric Food Chem        ISSN： 0021-8561            Impact factor:   5.279


  14 in total

1.  Synthesis and evaluation of 5-lipoxygenase translocation inhibitors from acylnitroso hetero-Diels-Alder cycloadducts.

Authors:  Joshua K Bolger; Wen Tian; William R Wolter; Wonhwa Cho; Mark A Suckow; Marvin J Miller
Journal:  Org Biomol Chem       Date:  2011-03-02       Impact factor: 3.876

2.  Cerumen of Australian stingless bees (Tetragonula carbonaria): gas chromatography-mass spectrometry fingerprints and potential anti-inflammatory properties.

Authors:  Flavia Carmelina Massaro; Peter Richard Brooks; Helen Margaret Wallace; Fraser Donald Russell
Journal:  Naturwissenschaften       Date:  2011-02-24

3.  Polyamine metabolism and lipoxygenase activity during Fusarium oxysporum f. sp. ricini -Castor interaction.

Authors:  Somnath D Mhaske; Mahesh Kumar Mahatma; Sanjay Jha; Pushpendra Singh; Taslim Ahmad
Journal:  Physiol Mol Biol Plants       Date:  2013-07

4.  Effect of cadmium and temperature on the lipoxygenase activity in barley root tip.

Authors:  Ladislav Tamás; Jana Dudíková; Katarína Durceková; L'ubica Halusková; Jana Huttová; Igor Mistrík
Journal:  Protoplasma       Date:  2008-12-06       Impact factor: 3.356

5.  Sph3 Is a Glycoside Hydrolase Required for the Biosynthesis of Galactosaminogalactan in Aspergillus fumigatus.

Authors:  Natalie C Bamford; Brendan D Snarr; Fabrice N Gravelat; Dustin J Little; Mark J Lee; Caitlin A Zacharias; Josée C Chabot; Alexander M Geller; Stefanie D Baptista; Perrin Baker; Howard Robinson; P Lynne Howell; Donald C Sheppard
Journal:  J Biol Chem       Date:  2015-09-04       Impact factor: 5.157

6.  Methods for identifying lipoxygenase producing microorganisms on agar plates.

Authors:  Antti Nyyssölä; Ruud Heshof; Thomas Haarmann; Jasmin Eidner; Ann Westerholm-Parvinen; Kim Langfelder; Kristiina Kruus; Leo de Graaff; Johanna Buchert
Journal:  AMB Express       Date:  2012-03-26       Impact factor: 3.298

7.  Phthalimide analogs as probable 15-lipoxygenase-1 inhibitors: synthesis, biological evaluation and docking studies.

Authors:  Alireza Aliabadi; Ahmad Mohammadi-Farani; Zeinab Hosseinzadeh; Hamid Nadri; Alireza Moradi; Farahnaz Ahmadi
Journal:  Daru       Date:  2015-07-22       Impact factor: 3.117

8.  Determination of Lipid Hydroperoxides in Marine Diatoms by the FOX2 Assay.

Authors:  Ida Orefice; Andrea Gerecht; Giuliana d'Ippolito; Angelo Fontana; Adrianna Ianora; Giovanna Romano
Journal:  Mar Drugs       Date:  2015-09-11       Impact factor: 5.118

9.  Lipoxygenase in Wheat: Genetic Control and Impact on Stability of Lutein and Lutein Esters.

Authors:  Daryl J Mares; Judy Cheong; Shashi N Goonetilleke; Diane E Mather
Journal:  Foods       Date:  2021-05-20

10.  Rare allele of HvLox-1 associated with lipoxygenase activity in barley (Hordeum vulgare L.).

Authors:  Ganggang Guo; Dawa Dondup; Xingmiao Yuan; Fanghong Gu; Deliang Wang; Fengchao Jia; Zhiping Lin; Michael Baum; Jing Zhang
Journal:  Theor Appl Genet       Date:  2014-09-12       Impact factor: 5.699
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