Literature DB >> 11169855

The beauty of the yeast: live cell microscopy at the limits of optical resolution.

S D Kohlwein1.   

Abstract

The yeast Saccharomyces cerevisiae is a very powerful system for cell biological research. Recent advances in electronic light microscopy together with the application of green fluorescent protein and other in vivo staining techniques have allowed novel and exciting insights into structural organization and dynamics of cells as small as yeast. Methods for staining yeast for microscopic inspection and for introducing tags for localization studies of proteins in living or fixed cells are summarized. Electronic light microscopy, video/deconvolution methods, and confocal laser scanning microscopy as novel tools for structural analyses, and their practical applications in yeast, are discussed. Copyright 2000 Wiley-Liss, Inc.

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Year:  2000        PMID： 11169855     DOI： 10.1002/1097-0029(20001215)51:6<511::AID-JEMT3>3.0.CO;2-Y

Source DB:  PubMed          Journal:  Microsc Res Tech        ISSN： 1059-910X            Impact factor:   2.769


  10 in total

1.  Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle.

Authors:  Brian L Sprague; Chad G Pearson; Paul S Maddox; Kerry S Bloom; E D Salmon; David J Odde
Journal:  Biophys J       Date:  2003-06       Impact factor: 4.033

2.  YPL.db: the Yeast Protein Localization database.

Authors:  Georg Habeler; Klaus Natter; Gerhard G Thallinger; Matthew E Crawford; Sepp D Kohlwein; Zlatko Trajanoski
Journal:  Nucleic Acids Res       Date:  2002-01-01       Impact factor: 16.971

3.  Fully hydrated yeast cells imaged with electron microscopy.

Authors:  Diana B Peckys; Peter Mazur; Kathleen L Gould; Niels de Jonge
Journal:  Biophys J       Date:  2011-05-18       Impact factor: 4.033

4.  SPO71 mediates prospore membrane size and maturation in Saccharomyces cerevisiae.

Authors:  Emily M Parodi; Crystal S Baker; Cayla Tetzlaff; Sasha Villahermosa; Linda S Huang
Journal:  Eukaryot Cell       Date:  2012-05-18

Review 5.  Review of methods to probe single cell metabolism and bioenergetics.

Authors:  Andreas E Vasdekis; Gregory Stephanopoulos
Journal:  Metab Eng       Date:  2014-10-31       Impact factor: 9.783

6.  Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae.

Authors:  S D Kohlwein; S Eder; C S Oh; C E Martin; K Gable; D Bacikova; T Dunn
Journal:  Mol Cell Biol       Date:  2001-01       Impact factor: 4.272

7.  Mechanistic study of the photodynamic inactivation of Candida albicans by a cationic porphyrin.

Authors:  S A G Lambrechts; M C G Aalders; J Van Marle
Journal:  Antimicrob Agents Chemother       Date:  2005-05       Impact factor: 5.191

8.  Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis.

Authors:  C Mo; M Valachovic; S K Randall; J T Nickels; M Bard
Journal:  Proc Natl Acad Sci U S A       Date:  2002-07-15       Impact factor: 11.205

9.  A heritable structural alteration of the yeast mitochondrion.

Authors:  Daniel Lockshon
Journal:  Genetics       Date:  2002-08       Impact factor: 4.562

Review 10.  The emergence of lipid droplets in yeast: current status and experimental approaches.

Authors:  Maja Radulovic; Oskar Knittelfelder; Alvaro Cristobal-Sarramian; Dagmar Kolb; Heimo Wolinski; Sepp D Kohlwein
Journal:  Curr Genet       Date:  2013-09-21       Impact factor: 3.886
  10 in total

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