| Literature DB >> 11169055 |
H J Brooks1, B D Mollison, K A Bettelheim, K Matejka, K A Paterson, V K Ward.
Abstract
Retail raw meat was sampled for the presence of Shiga toxin-producing Escherichia coli (STEC) using enrichment culture and Vero cell assay. The STEC obtained were serotyped and tested for enterohaemolysin (Ehly) production and the eae gene. The presence of Shiga toxin genes (stx) was confirmed by polymerase chain reaction. A total of 18 STEC were isolated accounting for 12% of beef, 17% of lamb and 4% of pork samples. Five isolates produced Ehly but none possessed the eae gene. Five isolates were identified which possessed the stx2 gene and belonged to serotypes associated with severe infection.Entities:
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Year: 2001 PMID: 11169055 DOI: 10.1046/j.1472-765x.2001.00868.x
Source DB: PubMed Journal: Lett Appl Microbiol ISSN: 0266-8254 Impact factor: 2.858