OBJECTIVE: To determine persistence and variability of colonization with Pseudomonas aeruginosa in cystic fibrosis patients over long time periods, and to look for possible cross-colonization. METHODS: In total, 469 Pseudomonas aeruginosa isolates were obtained from 30 patients during the period from April 1994 to April 1996. The sources were mainly sputum and a few deep throat swabs. All grown strains dissimilar in macromorphology were processed separately. Typing with PFGE was carried out by contour-clamped homogeneous electric field electrophoresis. Genomic DNA was subjected to the rare-cutting restriction enzyme SpeI. For pyocin typing, the procedure described by Fyfe was applied. RESULTS: After typing with PFGE, we observed 40 restriction profiles. Eighteen different pyocin types were found. The most frequent pyocin type was type 3, followed by types 1 and 5. Twenty-two patients were persistently colonized by one clone specific and different for each patient, and four were co-colonized by a second clone also different for each of these patients. Cross-colonization had apparently been rare in the cystic fibrosis center of Leipzig. CONCLUSIONS: Typing with PFGE is well suited for detailed investigations of colonization with Pseudomonas aeruginosa in cystic fibrosis patients. Pyocin typing can provide additional information for epidemiologic purposes.
OBJECTIVE: To determine persistence and variability of colonization with Pseudomonas aeruginosa in cystic fibrosispatients over long time periods, and to look for possible cross-colonization. METHODS: In total, 469 Pseudomonas aeruginosa isolates were obtained from 30 patients during the period from April 1994 to April 1996. The sources were mainly sputum and a few deep throat swabs. All grown strains dissimilar in macromorphology were processed separately. Typing with PFGE was carried out by contour-clamped homogeneous electric field electrophoresis. Genomic DNA was subjected to the rare-cutting restriction enzyme SpeI. For pyocin typing, the procedure described by Fyfe was applied. RESULTS: After typing with PFGE, we observed 40 restriction profiles. Eighteen different pyocin types were found. The most frequent pyocin type was type 3, followed by types 1 and 5. Twenty-two patients were persistently colonized by one clone specific and different for each patient, and four were co-colonized by a second clone also different for each of these patients. Cross-colonization had apparently been rare in the cystic fibrosis center of Leipzig. CONCLUSIONS: Typing with PFGE is well suited for detailed investigations of colonization with Pseudomonas aeruginosa in cystic fibrosispatients. Pyocin typing can provide additional information for epidemiologic purposes.
Authors: John E Moore; Colin E Goldsmith; J Stuart Elborn; Philip G Murphy; Peter H Gilligan; Séamus Fanning; Graham Hogg Journal: J Clin Microbiol Date: 2003-11 Impact factor: 5.948
Authors: Lilian Masoud-Landgraf; Alexandra Badura; Ernst Eber; Gebhard Feierl; Josefa Posch; Gernot Zarfel; Maximilian Zach; Egon Marth Journal: Wien Klin Wochenschr Date: 2012-04-21 Impact factor: 1.704
Authors: Johann D D Pitout; Barbara L Chow; Daniel B Gregson; Kevin B Laupland; Sameer Elsayed; Deirdre L Church Journal: J Clin Microbiol Date: 2006-11-22 Impact factor: 5.948