Literature DB >> 11166426

Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars.

K Datta1, J Tu, N Oliva, R Velazhahan, T W. Mew, S Muthukrishnan, S K. Datta.   

Abstract

Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T(2) generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani.

Entities:  

Year:  2001        PMID： 11166426     DOI： 10.1016/s0168-9452(00)00413-1

Source DB:  PubMed          Journal:  Plant Sci        ISSN： 0168-9452            Impact factor:   4.729


  44 in total

1.  Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice.

Authors:  Jianzhong Lin; Bo Zhou; Yuanzhu Yang; Jin Mei; Xiaoying Zhao; Xinhong Guo; Xingqun Huang; Dongying Tang; Xuanming Liu
Journal:  Plant Cell Rep       Date:  2009-05-20       Impact factor: 4.570

2.  Green tissue-specific co-expression of chitinase and oxalate oxidase 4 genes in rice for enhanced resistance against sheath blight.

Authors:  Subhasis Karmakar; Kutubuddin Ali Molla; Palas K Chanda; Sailendra Nath Sarkar; Swapan K Datta; Karabi Datta
Journal:  Planta       Date:  2015-09-08       Impact factor: 4.116

3.  A comparative proteomic approach to analyse structure, function and evolution of rice chitinases: a step towards increasing plant fungal resistance.

Authors:  Kishore Sarma; Budheswar Dehury; Jagajjit Sahu; Ranjan Sarmah; Smita Sahoo; Mousumi Sahu; Priyabrata Sen; Mahendra Kumar Modi; Madhumita Barooah
Journal:  J Mol Model       Date:  2012-06-09       Impact factor: 1.810

4.  Association between sheath blight resistance and chitinase activity in transgenic rice plants expressing McCHIT1 from bitter melon.

Authors:  Changwei Zhang; Manyu Huang; Xianchun Sang; Ping Li; Yinghua Ling; Fangming Zhao; Dan Du; Yunfeng Li; Zhenglin Yang; Guanghua He
Journal:  Transgenic Res       Date:  2019-06-18       Impact factor: 2.788

5.  Enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene RC24.

Authors:  Xuan Huang; Jian Wang; Zhen Du; Chen Zhang; Lan Li; Ziqin Xu
Journal:  Transgenic Res       Date:  2013-03-26       Impact factor: 2.788

6.  Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight.

Authors:  Ju-Kon Kim; In-Cheol Jang; Ray Wu; Wei-Neng Zuo; Rebecca S Boston; Yong-Hwan Lee; Il-Pyung Ahn; Baek Hie Nahm
Journal:  Transgenic Res       Date:  2003-08       Impact factor: 2.788

7.  The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco.

Authors:  M Carstens; M A Vivier; I S Pretorius
Journal:  Transgenic Res       Date:  2003-08       Impact factor: 2.788

8.  Agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptII gene as a plant selection marker.

Authors:  M Chakraborty; P Sairam Reddy; M Laxmi Narasu; Gaurav Krishna; Debashis Rana
Journal:  Physiol Mol Biol Plants       Date:  2016-02-04

9.  Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation.

Authors:  R Bevitori; M Popielarska-Konieczna; E M dos Santos; M F Grossi-de-Sá; S Petrofeza
Journal:  Protoplasma       Date:  2013-10-02       Impact factor: 3.356

10.  Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes.

Authors:  Hyun-Sun Ko; Rong-De Jin; Hari B Krishnan; Sang-Bog Lee; Kil-Yong Kim
Journal:  Curr Microbiol       Date:  2009-08-29       Impact factor: 2.188
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