Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence.

A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlox1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rlox1 lipoxygenase is involved in senescence of rose flowers.