Literature DB >> 11164306

Cloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isiB and its own promoter.

M T Bes1, J A Hernández, M L Peleato, M F Fillat.   

Abstract

A gene coding for a Fur (ferric uptake regulation) protein from the cyanobacterium Anabaena PCC 7119 has been cloned and overexpressed in Escherichia coli. DNA sequence analysis confirmed the presence of a 151-amino-acid open reading frame that showed homology with the Fur proteins reported for the unicellular cyanobacteria Synechococcus 7942 and Synechocystis PCC 6803. Two putative Fur-binding sites were detected in the promoter regions of the fur gene from Anabaena. Partially purified recombinant Fur binds to the flavodoxin promoter as well as its own promoter. This suggests that the Fur gene is autoregulated in Anabaena.

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Year:  2001        PMID： 11164306     DOI： 10.1111/j.1574-6968.2001.tb09467.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  8 in total

1.  Novel DNA-binding proteins in the cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  Olga A Koksharova; C Peter Wolk
Journal:  J Bacteriol       Date:  2002-07       Impact factor: 3.490

2.  The conformational stability and thermodynamics of Fur A (ferric uptake regulator) from Anabaena sp. PCC 7119.

Authors:  José A Hernández; Jörg Meier; Francisco N Barrera; Olga Ruiz de los Paños; Estefanía Hurtado-Gómez; M Teresa Bes; María F Fillat; M Luisa Peleato; Claudio N Cavasotto; José L Neira
Journal:  Biophys J       Date:  2005-09-16       Impact factor: 4.033

3.  Biochemical analysis of the recombinant Fur (ferric uptake regulator) protein from Anabaena PCC 7119: factors affecting its oligomerization state.

Authors:  José A Hernández; M Teresa Bes; María F Fillat; José L Neira; M Luisa Peleato
Journal:  Biochem J       Date:  2002-08-15       Impact factor: 3.857

4.  Unraveling the redox properties of the global regulator FurA from Anabaena sp. PCC 7120: disulfide reductase activity based on its CXXC motifs.

Authors:  Laura Botello-Morte; M Teresa Bes; Begoña Heras; Ángela Fernández-Otal; M Luisa Peleato; María F Fillat
Journal:  Antioxid Redox Signal       Date:  2014-01-02       Impact factor: 8.401

5.  Cysteine Mutational Studies Provide Insight into a Thiol-Based Redox Switch Mechanism of Metal and DNA Binding in FurA from Anabaena sp. PCC 7120.

Authors:  Laura Botello-Morte; Silvia Pellicer; Violeta C Sein-Echaluce; Lellys M Contreras; José Luis Neira; Olga Abián; Adrián Velázquez-Campoy; María Luisa Peleato; María F Fillat; María Teresa Bes
Journal:  Antioxid Redox Signal       Date:  2015-10-09       Impact factor: 8.401

6.  Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain.

Authors:  Fang Wang; Shuang Cheng; Kun Sun; Li Sun
Journal:  J Microbiol       Date:  2008-07-05       Impact factor: 3.422

7.  Periodic and coordinated gene expression between a diazotroph and its diatom host.

Authors:  Matthew J Harke; Kyle R Frischkorn; Sheean T Haley; Frank O Aylward; Jonathan P Zehr; Sonya T Dyhrman
Journal:  ISME J       Date:  2018-08-16       Impact factor: 10.302

8.  Genome-wide identification and characterization of Fur-binding sites in the cyanobacteria Synechocystis sp. PCC 6803 and PCC 6714.

Authors:  Matthias Riediger; Miguel A Hernández-Prieto; Kuo Song; Wolfgang R Hess; Matthias E Futschik
Journal:  DNA Res       Date:  2021-10-11       Impact factor: 4.477
  8 in total

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