F Bigonnesse1, Y Labelle, A Akoum. 1. Laboratoire d'Endocrinologie de la Reproduction, Centre Hospitalier Universitaire de Québec, Faculté de médecine, Université Laval, Québec, Québec, Canada.
Abstract
OBJECTIVE: To evaluate expression of interleukin-1 receptor type I in the endometrium of fertile women throughout the menstrual cycle and to investigate whether unexplained infertility may be associated with abnormal expression of IL-1 receptor type I. DESIGN: Retrospective study using immunohistochemical technique, Western blot assay, and reverse transcription polymerase chain reaction. SETTING: Gynecology clinic and human reproduction research laboratory. PATIENT(S): 39 fertile women and 25 women with unexplained infertility. INTERVENTION(S): Endometrial biopsy of samples obtained at laparoscopy. MAIN OUTCOME MEASURE(S): Immunostaining intensity, molecular weight, and messenger RNA levels of IL-1 receptor type I. RESULT(S): Immunostaining showed that two threshold days (13 and 22) separate the menstrual cycle into three distinct periods of IL-1 receptor type I expression, both in epithelial and stromal cells. Results of Western blot assay and reverse transcription polymerase chain reaction confirmed the immunohistochemical data. Statistical analyses showed that the pattern of IL-1 receptor type I expression was similar in women with unexplained infertility and fertile women. CONCLUSION(S): IL-1 receptor type I exhibits three distinct levels of expression throughout the menstrual cycle in the endometrium of fertile women, suggesting different physiologic roles of the receptor within the cycle. However, IL-1 receptor type I does not seem to be involved in unexplained infertility.
OBJECTIVE: To evaluate expression of interleukin-1 receptor type I in the endometrium of fertile women throughout the menstrual cycle and to investigate whether unexplained infertility may be associated with abnormal expression of IL-1 receptor type I. DESIGN: Retrospective study using immunohistochemical technique, Western blot assay, and reverse transcription polymerase chain reaction. SETTING: Gynecology clinic and human reproduction research laboratory. PATIENT(S): 39 fertile women and 25 women with unexplained infertility. INTERVENTION(S): Endometrial biopsy of samples obtained at laparoscopy. MAIN OUTCOME MEASURE(S): Immunostaining intensity, molecular weight, and messenger RNA levels of IL-1 receptor type I. RESULT(S): Immunostaining showed that two threshold days (13 and 22) separate the menstrual cycle into three distinct periods of IL-1 receptor type I expression, both in epithelial and stromal cells. Results of Western blot assay and reverse transcription polymerase chain reaction confirmed the immunohistochemical data. Statistical analyses showed that the pattern of IL-1 receptor type I expression was similar in women with unexplained infertility and fertile women. CONCLUSION(S): IL-1 receptor type I exhibits three distinct levels of expression throughout the menstrual cycle in the endometrium of fertile women, suggesting different physiologic roles of the receptor within the cycle. However, IL-1 receptor type I does not seem to be involved in unexplained infertility.
Authors: Koji Yoshinaga; Mercy PrabhuDas; Christopher Davies; Kenneth White; Kathleen Caron; Thaddeus Golos; Asgerally Fazleabas; Bibhash Paria; Gil Mor; Soumen Paul; Xiaoqin Ye; Sudhansu K Dey; Thomas Spencer; Robert Michael Roberts Journal: Am J Reprod Immunol Date: 2013-11-29 Impact factor: 3.886