| Literature DB >> 11157966 |
A Rincé1, J C Giard, V Pichereau, S Flahaut, Y Auffray.
Abstract
The Enterococcus faecalis general stress protein Gsp65 has been purified from two-dimensional gel electrophoresis. Determination of its N-terminal sequence and characterization of the corresponding gene revealed that the gsp65 product is a 133-amino-acid protein sharing homologies with organic hydroperoxide resistance (Ohr) proteins. Transcriptional analysis of gsp65 gave evidence for a monocistronic mRNA initiated 52 nucleotides upstream of the ATG start codon and for an induction in response to hydrogen peroxide, heat shock, acid pH, detergents, ethanol, sodium chloride, and tert-butylhydroperoxide (tBOOH). A gsp65 mutant showed increased sensitivity to the organic hydroperoxide tBOOH and to ethanol.Entities:
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Year: 2001 PMID: 11157966 PMCID: PMC95027 DOI: 10.1128/JB.183.4.1482-1488.2001
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490