Literature DB >> 11157966

Identification and characterization of gsp65, an organic hydroperoxide resistance (ohr) gene encoding a general stress protein in Enterococcus faecalis.

A Rincé1, J C Giard, V Pichereau, S Flahaut, Y Auffray.   

Abstract

The Enterococcus faecalis general stress protein Gsp65 has been purified from two-dimensional gel electrophoresis. Determination of its N-terminal sequence and characterization of the corresponding gene revealed that the gsp65 product is a 133-amino-acid protein sharing homologies with organic hydroperoxide resistance (Ohr) proteins. Transcriptional analysis of gsp65 gave evidence for a monocistronic mRNA initiated 52 nucleotides upstream of the ATG start codon and for an induction in response to hydrogen peroxide, heat shock, acid pH, detergents, ethanol, sodium chloride, and tert-butylhydroperoxide (tBOOH). A gsp65 mutant showed increased sensitivity to the organic hydroperoxide tBOOH and to ethanol.

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Year:  2001        PMID: 11157966      PMCID: PMC95027          DOI: 10.1128/JB.183.4.1482-1488.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  30 in total

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5.  Unusual resistance and acquired tolerance to cadmium chloride in Enterococcus faecalis.

Authors:  J M Laplace; P Boutibonnes; Y Auffray
Journal:  J Basic Microbiol       Date:  1996       Impact factor: 2.281

6.  Comparison of the bile salts and sodium dodecyl sulfate stress responses in Enterococcus faecalis.

Authors:  S Flahaut; J Frere; P Boutibonnes; Y Auffray
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7.  Recombination-deficient mutant of Streptococcus faecalis.

Authors:  Y Yagi; D B Clewell
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8.  Glucose starvation response in Enterococcus faecalis JH2-2: survival and protein analysis.

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Authors:  P Boutibonnes; J C Giard; A Hartke; B Thammavongs; Y Auffray
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  28 in total

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Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

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3.  The response regulator CroR modulates expression of the secreted stress-induced SalB protein in Enterococcus faecalis.

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4.  The msaABCR Operon Regulates the Response to Oxidative Stress in Staphylococcus aureus.

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5.  Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr.

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Review 6.  Adaptation to Adversity: the Intermingling of Stress Tolerance and Pathogenesis in Enterococci.

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9.  Comparative genomic analysis of pathogenic and probiotic Enterococcus faecalis isolates, and their transcriptional responses to growth in human urine.

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10.  The transcriptome of the nosocomial pathogen Enterococcus faecalis V583 reveals adaptive responses to growth in blood.

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