Literature DB >> 1115794

A new principle in biospecific affinity chromatography used for purification of cobalamin-binding proteins.

E Nexo.   

Abstract

To avoid using protein-denaturing agents for desorption, when purifying cobalamin-binding protein by biospecific affinity chromatography, an affinity column has been prepared where cobalamin is attached through a temperature-labile linkage to insolubilized 3, 3'-diaminodipropylamine. On desorption the protein is obtained in solution saturated with cobalamin. The method has been used for purification of intrinsic factor and transcobalamin I.

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Year:  1975        PMID： 1115794

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  The extracellular heme-binding protein HbpS from the soil bacterium Streptomyces reticuli is an aquo-cobalamin binder.

Authors:  Darío Ortiz de Orué Lucana; Sergey N Fedosov; Ina Wedderhoff; Edith N Che; Andrew E Torda
Journal:  J Biol Chem       Date:  2014-10-23       Impact factor: 5.157

2.  Purification by cobalamin-Sepharose affinity chromatography and intrinsic factor-binding activity of an extramembrane proteolytic product from pig ileal mucosa.

Authors:  A Yerima; A Safi; I Gastin; J C Michalski; M Saunier; J L Gueant
Journal:  Biochem J       Date:  1996-01-15       Impact factor: 3.857

3.  Megalin-mediated endocytosis of transcobalamin-vitamin-B12 complexes suggests a role of the receptor in vitamin-B12 homeostasis.

Authors:  S K Moestrup; H Birn; P B Fischer; C M Petersen; P J Verroust; R B Sim; E I Christensen; E Nexø
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

4.  A single rainbow trout cobalamin-binding protein stands in for three human binders.

Authors:  Eva Greibe; Sergey Fedosov; Boe S Sorensen; Peter Højrup; Steen S Poulsen; Ebba Nexo
Journal:  J Biol Chem       Date:  2012-08-07       Impact factor: 5.157

5.  Effect of the vitamin B12-binding protein haptocorrin present in human milk on a panel of commensal and pathogenic bacteria.

Authors:  Henrik R Jensen; Martin F Laursen; Dorte L Lildballe; Jens B Andersen; Ebba Nexø; Tine R Licht
Journal:  BMC Res Notes       Date:  2011-06-21

6.  Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.

Authors:  Katrine Hygum; Dorte L Lildballe; Eva H Greibe; Anne L Morkbak; Steen S Poulsen; Boe S Sorensen; Torben E Petersen; Ebba Nexo
Journal:  PLoS One       Date:  2011-05-31       Impact factor: 3.240

7.  The cobalamin-binding protein in zebrafish is an intermediate between the three cobalamin-binding proteins in human.

Authors:  Eva Greibe; Sergey Fedosov; Ebba Nexo
Journal:  PLoS One       Date:  2012-04-20       Impact factor: 3.240

8.  Comparison of recombinant human haptocorrin expressed in human embryonic kidney cells and native haptocorrin.

Authors:  Evelyne Furger; Sergey N Fedosov; Dorte Launholt Lildballe; Robert Waibel; Roger Schibli; Ebba Nexo; Eliane Fischer
Journal:  PLoS One       Date:  2012-05-25       Impact factor: 3.240

9.  Development of a Sensitive ELISA for Gastric Intrinsic Factor and Detection of Intrinsic Factor Immunoreactivity in Human Serum.

Authors:  Eva Greibe; Ebba Nexo
Journal:  Nutrients       Date:  2022-09-28       Impact factor: 6.706
  9 in total

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