Literature DB >> 11157270

Production of capsular polysaccharide of Streptococcus pneumoniae type 14 and its purification by affinity chromatography.

N Suárez1, L F Fraguas, E Texeira, H Massaldi, F Batista-Viera, F Ferreira.   

Abstract

We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.

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Year:  2001        PMID： 11157270      PMCID： PMC92674          DOI： 10.1128/AEM.67.2.969-971.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

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Journal:  Microb Drug Resist       Date:  1997       Impact factor: 3.431

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Journal:  Eur J Biochem       Date:  1994-04-01

7.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365
  7 in total
  4 in total

1.  Sialylation of group B streptococcal capsular polysaccharide is mediated by cpsK and is required for optimal capsule polymerization and expression.

Authors:  D O Chaffin; L M Mentele; C E Rubens
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

2.  Enhanced Production of Shigella flexneri Polysaccharide from a Newly Devised Silicate Method as a Potential Vaccine Conjugate.

Authors:  P V S L S S Narayana; Jayati Ray Dutta
Journal:  Curr Microbiol       Date:  2019-10-03       Impact factor: 2.188

3.  Development and evaluation of a novel vaccine against prevalent invasive multi-drug resistant strains of Streptococcus pneumoniae.

Authors:  Rehab H Bahy; Hayam M Hamouda; Amal S Shahat; Aymen S Yassin; Magdy A Amin
Journal:  PeerJ       Date:  2016-11-30       Impact factor: 2.984

Review 4.  Purification of Capsular Polysaccharides of Streptococcus pneumoniae: Traditional and New Methods.

Authors:  Victor Morais; Valerie Dee; Norma Suárez
Journal:  Front Bioeng Biotechnol       Date:  2018-10-12
  4 in total

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